Showing papers by "Chandana Haldar published in 2020"
TL;DR: Melatonin treatment remarkably restored testicular function as evident by normal histoarchitecture, increased sperm count/viability, enhanced antioxidant enzyme activities (SOD and Catalase), and decreased lipid peroxidation (LPO) level.
Abstract: The excess fluoride intake has been shown to adversely affect male reproductive health. The aim of the present study was to investigate the key mechanism underlying fluoride-induced testicular dysfunction and the role of melatonin as a modulator of testicular metabolic, oxidative, and inflammatory load. The present results indicated that sodium fluoride (NaF) exposure to adult male golden hamsters severely impairs reproductive physiology as evident from markedly reduced sperm count/viability, testosterone level, androgen receptor (AR), testicular glucose transporter (GLUT-1), gap junction (connexin-43), and survival (Bcl-2) protein expression. NaF exposure markedly increased testicular oxidative load, inflammatory (NF-kB/COX-2), and apoptotic (caspase-3) protein expression. However, melatonin treatment remarkably restored testicular function as evident by normal histoarchitecture, increased sperm count/viability, enhanced antioxidant enzyme activities (SOD and Catalase), and decreased lipid peroxidation (LPO) level. In addition, melatonin treatment upregulated testicular Nrf-2/HO-I, SIRT-1/ FOXO-1, and downregulated NF-kB/COX-2 expression. Further, melatonin ameliorated NaF-induced testicular metabolic stress by modulating testicular GLUT-1expression, glucose level, and LDH activity. Furthermore, melatonin treatment enhanced testicular PCNA, Bcl-2, connexin-43, and reduced caspase-3 expression. In conclusion, we propose the molecular mechanism of fluoride-induced testicular damages and ameliorative action(s) of melatonin.
17 citations
12 citations
TL;DR: The results suggest that even in tropical rodents seasonal variation in photoperiod is responsible for initiating female reproductive activities where circulatory melatonin via MT1R plays an important role.
Abstract: To elucidate the role of photoperiodically modulated endogenous melatonin and its receptor MT1 in the ovary of a diurnal rodent, adult Indian palm squirrel Funambulus pennanti were exposed ...
8 citations
TL;DR: It can be inferred that photoperiod modulates the circulatory oestrogen level, along with gap junction protein and apoptosis markers expression in testes and harmonizes the energy homeostasis involved in reproductive maintenance for seasonality of golden hamster.
Abstract: The environmental photoperiod plays an important role in regulation of seasonal reproductive events. However, with reference tooestrogen status the exact mechanism involved in testicular seasonalit...
5 citations
TL;DR: The results suggest that the antibiotic induced nephrotoxicity can be ameliorated by melatonin or Vit-C via reduction of oxidative stress in renal tissues.
Abstract: Ampicillin is one of the most commonly prescribed antibiotics against intra-renal infections. Prolonged use of ampicillin has been found to be associated with a high rate of free-radical ge...
3 citations
01 Jan 2020
TL;DR: In this article, different concentrations of melatonin and PLA were prepared by dissolving in dichloromethane (DCM), and the final dried nanoparticles were used for structural analysis by SEM, TEM, and FTIR.
Abstract: Melatonin-loaded PLA nanoparticles are nowadays important in biological system for its biodegradable nature for targeted drug delivery. Hence, aim of the study is to note applicability and toxicity of Mel-PLA nanoparticles in osteoporosis. Different concentrations of melatonin and PLA were prepared by dissolving in dichloromethane (DCM). The final dried nanoparticles were used for structural analysis by SEM, TEM, and FTIR. Toxicity and immunological impact of nanoparticles were evaluated on rats: control and nanoparticle treated (n = 5/group) for 7 days. Afterward animals were sacrificed and blood, liver, and kidney were collected. A fraction of blood was processed for TLC, DLC, and %LC, and the remaining was centrifuged at 3000x g at 4 °C for 30 min. Separated plasma was used for measurements of IL-2, IL-6, TNF-α, IFN-γ, IL-1β, urea, creatinine, and BUN. Both plasma and tissue homogenates were used for AST, ALT, ACP, and ALP estimations. We noted significantly high (p 0.05) or unaffected (IFN-γ, ACP, and ALP). From our preliminary study, we may conclude that we have synthesized Mel-PLA nanoparticles and their effects were nontoxic to animals.
2 citations
TL;DR: The results suggest that a trade‐off relationship between melatonin and testosterone exists in regulation of their receptors in lung of Perdicula asiatica.
Abstract: The role of circulatory steroid hormone along with melatonin in lung of any seasonally breeding bird has never been explored so far. This could be interesting because steroid hormones are immunosuppressive while melatonin is immunostimulatory in nature. In our present study, we report the effect of exogenous melatonin and testosterone on expression of melatonin receptor subtypes (Mel1a and Mel1b ) and androgen receptor in lung of a tropical bird Perdicula asiatica. Birds were collected from vicinity of Varanasi and acclimatized in laboratory with sufficient food and water. The birds were treated with melatonin and testosterone at dose of 25 µg/100 g B.wt./day and 1 mg/100 g B.wt./day, respectively, for 28 days. At the end of the experiment, the birds were sacrificed and lung tissue and blood sample were collected for immunohistochemistry, Western blot analysis and hormonal assay. Testosterone treatment increased circulatory testosterone and upregulated expression of androgen receptors whereas downregulated expression of melatonin receptor subtypes Mel1a and Mel1b . Melatonin administration increased peripheral melatonin and upregulated expression of melatonin receptor subtypes Mel1a and Mel1b while downregulated androgen receptor. Thus, our results suggest that a trade-off relationship between melatonin and testosterone exists in regulation of their receptors in lung of Perdicula asiatica.
2 citations
1 citations
TL;DR: The role of glucocorticoid and melatonin receptors in the regulation of lung-associated cell-mediated immunity has been explored in any tropical bird as mentioned in this paper, where the birds were treated with melatonin and dexamethasone.
Abstract: The role of circulatory glucocorticoids along with melatonin
in the regulation of lung-associated cell-mediated immunity has
never been explored in any tropical bird. This could be interesting because
glucocorticoids are immunosuppressive while melatonin is immunostimulatory
in nature. In our present study, we report the localization and
expression of the glucocorticoid and melatonin receptors and the
effect of melatonin on dexamethasone-induced suppression of lung-associated and
general immunity in the jungle bush quail Perdicula
asiatica. The birds were treated with melatonin and dexamethasone
at dose of 25µg/100g B.wt./day and 30µg/100g B.wt./day, respectively,
for twenty eight days. At the end of the experiment, the birds were
sacrificed and lung tissues and blood samples were collected for
histology and morphometric analysis of lymphoid tissue (BALT and
non-BALT nodules), total leukocyte count (TLC), lymphocyte count
(LC), percent stimulation ratio of isolated lung lymphocytes (%
SR), immunohistochemistry, western blot analysis and hormonal assay.
Dexamethasone injection reduced the immune status in terms of the
size of BALT and non-BALT nodules, TLC, LC and % SR, upregulated
expression of the glucocorticoid receptor (GR) and downregulated
expression of melatonin receptor types Mel1a and Mel1b.
Melatonin administration increased the above immune parameters and
upregulated expression of melatonin receptor types Mel1a and
Mel1b while downregulating GR. Our results suggest
the existence of the trade-off relationship between melatonin and
corticosterone, which might be responsible for the regulation of
lung-associated cell-mediated immunity under stress conditions via
their receptors in the lung of the jungle bush quail Perdicula asiatica.