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Chathura S. Abeywickrama

Bio: Chathura S. Abeywickrama is an academic researcher from University of Akron. The author has contributed to research in topics: Fluorescence & Stokes shift. The author has an hindex of 9, co-authored 23 publications receiving 292 citations.

Papers
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Journal ArticleDOI
TL;DR: An NIR-emitting probe with a large Stokes shift is synthesized by using excited-state intramolecular proton transfer (ESIPT), leading to a lysosome probe without exhibiting "an alkalinizing effect".

122 citations

Journal ArticleDOI
TL;DR: A highly fluorescent red-emitting pyrene derivative was synthesized and found to exhibit a large Stokes shift, and the probe molecule showed remarkable selectivity to stain the nucleus in both live and fixed cells, with higher sensitivity than commercial dye DRAQ5.

68 citations

Journal ArticleDOI
04 Mar 2021
TL;DR: In this article, the authors summarize the recent progress in the design and application of novel organic sensors with emission in the near-infrared region (600-900 nm) by coupling different functional groups with excited-state intramolecular proton transfer (ESIPT) segments.
Abstract: In this review, we will summarize our recent progress in the design and application of novel organic sensors with emission in the near-infrared region (600-900 nm). By coupling different functional groups with excited-state intramolecular proton transfer (ESIPT) segments, new probes are developed to achieve a large Stokes shift, high sensitivity, and selectivity and to tune the emission toward the near-infrared region. The developed probes exhibit attractive optical properties for bioimaging and environmental science applications. In addition, we further discuss the photophysical properties of ESIPT dyes and how their fluorescence could be affected by structural/environmental factors, which should be considered during the development of robust ESIPT-based fluorescence probes. Their potential applications as imaging reagents are illustrated for intracellular membranes, mitochondria, lysosomes, and some biomolecules.

53 citations

Journal ArticleDOI
TL;DR: A bright red-emitting flavonoid derivative was synthesized, generating a large bathochromic shift in both absorption and fluorescence to enable ratiometric determination of cellular Al3+.

33 citations

Journal ArticleDOI
TL;DR: A series of pyrene-benzothiazolium probes were synthesized that do not require proton participation and exhibit high biocompatibility and long-term imaging ability.

31 citations


Cited by
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Journal ArticleDOI
TL;DR: This review will explore recent advances in the design and application of excited-state intramolecular proton-transfer (ESIPT) based fluorescent probes.
Abstract: In this review we will explore recent advances in the design and application of excited-state intramolecular proton-transfer (ESIPT) based fluorescent probes. Fluorescence based sensors and imaging agents (probes) are important in biology, physiology, pharmacology, and environmental science for the selective detection of biologically and/or environmentally important species. The development of ESIPT-based fluorescence probes is particularly attractive due to their unique properties, which include a large Stokes shift, environmental sensitivity and potential for ratiometric sensing.

844 citations

Journal ArticleDOI
TL;DR: This review introduces the AIE concept and working principles of AIE bioprobes, and briefly summarizes their applications in biosensing and bioimaging with some representative examples recently reported mainly in the authors' group.
Abstract: Organic fluorescent bioprobes provide robust and powerful analytical techniques for direct detection and monitoring of important biological species and their related biological processes in live samples. In contrast to conventional fluorophores with aggregation-caused quenching effect, aggregation-induced emission (AIE) luminogens (AIEgens) exhibit remarkable photophysical properties such as bright fluorescence in aggregate or solid state, large Stokes shift, excellent photostability, and long retention time in live samples. Benefiting from these advantages, AIEgen-based bioprobes have been widely applied in diverse biomedical applications. In this review, we first introduce the AIE concept and working principles of AIE bioprobes. Then we briefly summarize their applications in biosensing and bioimaging with some representative examples recently reported mainly in our group. The challenge and future development of AIE bioprobes are also discussed and highlighted. Hopefully, this review can inspire more researchers to participate in this fascinating area and expand the versatile biomedical applications.

102 citations

Journal ArticleDOI
TL;DR: A new type of highly photoluminescent (PL) emerald carbon dots (CDs) was easily prepared through a functional preservation strategy (FPS) by simply mixing p-benzoquinone and ethanediamine at room temperature and could successfully monitor lysosomal pH dynamics during apoptosis in live cells.
Abstract: Lysosomes, which can be easily targeted by molecules with abundant amino groups, play critical roles in endocytosis, autophagy, and phagocytosis; thus, it is important to accurately characterize lysosomes, including lysosomal pH, in living cells to understand their physiological and pathological functions Herein, a new type of highly photoluminescent (PL) emerald carbon dots (CDs) was easily prepared through a functional preservation strategy (FPS) by simply mixing p-benzoquinone and ethanediamine at room temperature The as-prepared CDs possessed abundant amino groups preserved from ethanediamine owing to FPS, and they exhibited excellent photostability as compared to the commercial LysoTracker probes Consequently, they actively targeted lysosomes to sensitively respond to lysosomal pH in vitro owing to their abundant amino groups and good hydrophilicity Thus, we could successfully monitor lysosomal pH dynamics during apoptosis in live cells

81 citations

Journal Article
TL;DR: In this article, the two-photon fluorescence excitation (TPE) spectra were measured for 11 common molecular fluorophores in the excitation wavelength range 690 nm < λ < 1050 nm.
Abstract: Measurements of two-photon fluorescence excitation (TPE) spectra are presented for 11 common molecular fluorophores in the excitation wavelength range 690 nm < λ < 1050 nm. Results of excitation by ∼100-fs pulses of a mode-locked Ti:sapphire laser are corroborated by single-mode cw Ti:sapphire excitation data in the range 710 nm < λ < 840 nm. Absolute values of the TPE cross section for Rhodamine B and Fluorescein are obtained by comparison with one-photon-excited fluorescence, assuming equal emission quantum efficiencies. TPE action cross sections for the other nine fluorophores are also determined. No differences between one-photon- and two-photon-excited fluorescence emission spectra are found. TPE emission spectra are independent of excitation wavelength. With both pulsed and cw excitation the fluorescence emission intensities are strictly proportional to the square of the excitation intensity to within ±4% for excitation intensities sufficiently below excited-state saturation.

79 citations

Journal ArticleDOI
TL;DR: The recent advances in the design and preparation of small-molecule fluorescent probes for specific detection of chemical species inside lysosomes and their properties and applications for the detection and imaging are summarized.

78 citations