Cloning and characterization of the

https://www.cell.com/trends/biotechnology/pdf/S0167-7799(16)30028-2.pdf

Electro-Fermentation: How To Drive Fermentation Using Electrochemical Systems.

Redox potential control and applications in microaerobic and anaerobic fermentations

Bioelectrocatalysis is an interdisciplinary research field combining biocatalysis and electrocatalysis via the utilization of materials derived from biological systems as catalysts to catalyze the redox reactions occurring at an electrode. Bioelectrocatalysis synergistically couples the merits of both biocatalysis and electrocatalysis. The advantages of biocatalysis include high activity, high selectivity, wide substrate scope, and mild reaction conditions. The advantages of electrocatalysis include the possible utilization of renewable electricity as an electron source and high energy conversion efficiency. These properties are integrated to achieve selective biosensing, efficient energy conversion, and the production of diverse products. This review seeks to systematically and comprehensively detail the fundamentals, analyze the existing problems, summarize the development status and applications, and look toward the future development directions of bioelectrocatalysis. First, the structure, function, and modification of bioelectrocatalysts are discussed. Second, the essentials of bioelectrocatalytic systems, including electron transfer mechanisms, electrode materials, and reaction medium, are described. Third, the application of bioelectrocatalysis in the fields of biosensors, fuel cells, solar cells, catalytic mechanism studies, and bioelectrosyntheses of high-value chemicals are systematically summarized. Finally, future developments and a perspective on bioelectrocatalysis are suggested.

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis.

Engineering biological systems using automated biofoundries.

Production and characterization of okara dietary fiber produced by fermentation with Monascus anka.

Production of itaconic acid by biotransformation of wheat bran hydrolysate with Aspergillus terreus CICC40205 mutant

Effects of culture redox potential on succinic acid production by Corynebacterium crenatum under anaerobic conditions

Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria. To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the metabolic flux analysis were compared in the engineered strain and the original one. The acetic acid production was improved by the engineered strain (61.42 g L-1) while the residual ethanol content (4.18 g L-1) was decreased. Analysis of 2D maps indicated that 19 proteins were differently expressed between the two strains; of these, 17 were identified and analyzed by mass spectrometry and two-dimensional gel electrophoresis. With further investigation of metabolic flux analysis of the pathway from ethanol and glucose, the results reveal that over-expression of PQQ-ADH is an effective way to improve the ethanol oxidation respiratory chain pathway and these can offer theoretical references for potential mechanism of metabolic regulation in acetic acid bacteria and researches with its acetic acid resistance.

/pdf/improving-acetic-acid-production-by-over-expressing-pqq-adh-14j8e00sl8.pdf

Improving Acetic Acid Production by Over-Expressing PQQ-ADH in Acetobacter pasteurianus.

The invention relates to a method for producing L-lactic acid. In the method, crop straw and Rhizopus oryzae serve as raw materials. The method particularly comprises the following operation steps: A. preparing straw saccharified liquid; B. adding auxiliary material to inoculate Rhizopus oryzae for fermentation; C repeatedly fermenting 25 batches; D. acidizing fermented clear liquid; E. ultrafiltration; F. nanofilteration; G. reverse osmosis; H. ion exchange; I. concentrating in vacuum to obtain the L-lactic acid product with the mass percentage concentration of 85%. The invention takes the straw hydrolysis saccharified liquid as the main raw material to prepare L-lactic acid, which obviously lowers the production cost of L-lactic acid; Rhizopus oryzae is adopted for once inoculation proliferation, thallus can be reused for several times to produce L-lactic acid, thus shortening L-lactic acid fermentation period, and obviously improving the fermentation strength of L-lactic acid; an advanced membrane separation and ion exchange integration technology is adopted to improve the lactic acid product quality; and the invention has simple process and strong continuity.

Chen Xiaoju

Papers

Production and characterization of okara dietary fiber produced by fermentation with Monascus anka.

Production of itaconic acid by biotransformation of wheat bran hydrolysate with Aspergillus terreus CICC40205 mutant

Effects of culture redox potential on succinic acid production by Corynebacterium crenatum under anaerobic conditions

Improving Acetic Acid Production by Over-Expressing PQQ-ADH in Acetobacter pasteurianus.

Method for producing L-lactic acid