Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.

Method of sequencing a nucleic acid

Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

Method and apparatus for detection of fluorescently labeled materials

Apparatus and method for sequencing a nucleic acid

Systems and methods for aligning scanned images are provided. A pattern is included in the scanned image so that when the image is convolved with a filter, a recognizable pattern is generated in the convolved image. The scanned image may then be aligned according to the position of the recognizable pattern in the convolved image. The filter may also act to remove the portions of the scanned image that do not correspond to the pattern in the scanned image.

Scanned image alignment systems and methods

The present invention provides methods for comparing and identifying differences in nucleic acid sequences using a plurality of sequence specific recognition reagents (i.e., probes comprising a nucleic acid complementary to a nucleic acid sequence in collections to be compared) bound to a solid surface.

Method for comparing copy number of nucleic acid sequences

This invention provides oligonucleotide-based arrays and methods for speciating and phenotyping organisms, for example, using oligonucleotide sequences based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns in a database.

Chip-based speciation and phenotypic characterization of microorganisms

This invention provides oligonucleotide based arrays and methods for speciating and phenotyping organisms, for example, using oligonucleotide sequences based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns in a database.

Chip-based species identification and phenotypic characterization of microorganisms

Bioarray chip reaction apparatus and its manufacture

Speziesbestimmung und phenotypische charakterisierung von mikroorganismen auf der basis eines chips

L'invention concerne des ensembles a base d'oligonucleotides, ainsi que des procedes pour determiner les especes et les phenotypes d'organismes, par exemple, a l'aide de sequences d'oligonucleotides basees sur le gene Mycobacterium tuberculosis rpoB. Les groupes ou les especes auxquels appartient un organisme peuvent etre determines par comparaison de schemas d'hybridation d'acide nucleique cible provenant de l'organisme avec des schemas d'hybridation conserves dans une base de donnees.

Ching Wang

Papers

Chip-based speciation and phenotypic characterization of microorganisms

Chip-based species identification and phenotypic characterization of microorganisms

Bioarray chip reaction apparatus and its manufacture

Speziesbestimmung und phenotypische charakterisierung von mikroorganismen auf der basis eines chips

Spéciation de micro-organismes à partir de micro plaquettes et caractérisation des phénotypes de ceux-ci