Christine L. Gatlin

TL;DR: This approach succeeded in resolving approximately 3700 distinct protein spots, many of them post‐translationally modified variants of plasma proteins, and holds promise to accelerate the discovery of novel serum protein biomarkers.

TL;DR: The immunoaffinity‐based protein subtraction chromatography (IASC) described here removes multiple proteins present in plasma and serum in high concentrations effectively and reproducibly and facilitates automated chromatographic processing of plasma samples in high throughput, desirable in proteomic disease marker discovery projects.

TL;DR: A protein fractionation strategy enriching proteins of molecular masses lower than 30 kDa in a fraction separate from larger proteins is described, which led to the successful identification of 30% of the proteins.

TL;DR: A novel semiquantitative method is described to determine abundance factors of proteins in 2‐DE gels of cell envelope fractions relative to whole cell lysates and discusses these data in the context of true cell envelope localization versus experimentally caused cell lysis.

TL;DR: New insights are presented into liver gene network regulations induced by lovastatin and a yet unexplored application of proteomics to discover new targets by analysis of existing drugs and the pathways that they regulate is illustrated.