Showing papers by "Christopher J. Frederickson published in 1992"
TL;DR: The major cytoarchitectonic regions of the rat brain that stain with the Timm-Danscher metal stain were tested with the fluorescent probe for zinc, 6-methoxy 8-para toluene sulfonamide quinoline (TSQ), and it was concluded that theTimm- Danscher staining generally indicates zinc-containing axonal boutons.
108 citations
TL;DR: Among the tested compounds, sodium selenide (Na2Se) caused insignificant necrosis within the injection site and was easily transported retrogradely when handled anaerobically, and is recommended as the compound of choice.
Abstract: The autometallographic retrograde tracing of zinc-containing neurons by intracerebral injection of sodium selenite (Na2SeO3), introduced by Danscher in 1982, has recently been described in more detail. Intracerebral injections of both sodium selenide (Na2Se) and sodium selenite (Na2SeO3) have been successfully used; however, sodium selenite had a rather toxic effect on the injected tissue. In the present study, we tested seven different selenium compounds to find the most suitable compound for retrograde tracing of zinc-positive pathways. Among the tested compounds, sodium selenide (Na2Se) caused insignificant necrosis within the injection site and was easily transported retrogradely when handled anaerobically. Sodium selenide is therefore recommended as the compound of choice.
37 citations