D
Daniel N. Miller
Researcher at Agricultural Research Service
Publications - 83
Citations - 3185
Daniel N. Miller is an academic researcher from Agricultural Research Service. The author has contributed to research in topics: Manure & Feedlot. The author has an hindex of 26, co-authored 79 publications receiving 2902 citations. Previous affiliations of Daniel N. Miller include United States Department of Agriculture & University of Nebraska–Lincoln.
Papers
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Evaluation and Optimization of DNA Extraction and Purification Procedures for Soil and Sediment Samples
TL;DR: It is indicated that for these types of samples, optimum DNA recovery requires brief, low-speed bead mill homogenization in the presence of a phosphate-buffered SDS-chloroform mixture, followed by Sephadex G-200 column purification.
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Ammonium transport and reaction in contaminated groundwater: Application of isotope tracers and isotope fractionation studies
TL;DR: In this paper, a combination of techniques including large-scale monitoring of NH4+ distribution; isotopic analyses of coexisting aqueous NH4+, NO3−, N2, and sorbed NH4+); and in situ natural gradient 15NH4+ tracer tests with numerical simulations were studied.
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Distribution and Quantification of Antibiotic Resistant Genes and Bacteria across Agricultural and Non-Agricultural Metagenomes
TL;DR: Since antibiotic resistance genes are a natural part of both human-impacted and pristine habitats, presence of these resistance genes in any specific habitat is therefore not sufficient to indicate or determine impact of anthropogenic antibiotic use.
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Evaluation of gel filtration resins for the removal of PCR-inhibitory substances from soils and sediments.
TL;DR: A variety of gel filtration resins were evaluated for their efficacy in removing PCR-inhibitory substances from feedlot soil DNA crude extracts using gravity-flow disposable columns and Sepharose 2B was the most efficient purification resin based upon flow rate and the elution of DNA and humic acids from the columns.
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Cattle feedlot soil moisture and manure content: II. Impact on Escherichia coli O157.
Elaine D. Berry,Daniel N. Miller +1 more
TL;DR: E. coli O157 can persist and may even grow in feedlot soils, over a wide range of water and manure contents, and further investigations are needed to determine if these variables can be manipulated to reduce this pathogen in cattle and the feedlot environment.