Daniel S. Pilch

TL;DR: This work describes here the first systematic development of the novel aminoglycoside 2 (NB54) exhibiting superior in vitro readthrough efficiency to that of gentamicin in seven different DNA fragments derived from mutant genes carrying nonsense mutations representing the genetic diseases Usher syndrome, cystic fibrosis, Duchenne muscular dystrophy, and Hurler syndrome.

TL;DR: Spectroscopic and thermal denaturation data revealed that the crosslink alters the structure of the host duplex; lowers its thermal stability; and reduces its thermodynamic stability, but it does not alter the two-state melting behavior exhibited by the parent, unmodified duplex despite the significant crosslink-induced changes noted above.

TL;DR: Imino proton NMR spectra provide evidence for the existence of new purine-purine (pur.pur) hydrogen bonds, in addition to those of the Watson-Crick (W-C) base pairs, in the triplex structure.

TL;DR: The results reveal the impact of specific alterations in aminoglycoside structure on the thermodynamics of binding to an A-site model RNA oligonucleotide and the salt dependencies of the RNA binding affinities of neomycin and paromomycin are consistent with at least three drug NH(3)(+) groups participating in electrostatic interactions with the host RNA.

TL;DR: The notable differences observed between nonquaternized and quaternized quinoxaline derivatives suggest that differing mechanisms of action are associated with their antibacterial properties.