D
Darren D. Sledjeski
Researcher at University of Toledo Medical Center
Publications - 26
Citations - 2757
Darren D. Sledjeski is an academic researcher from University of Toledo Medical Center. The author has contributed to research in topics: Streptococcus pyogenes & rpoS. The author has an hindex of 19, co-authored 26 publications receiving 2708 citations. Previous affiliations of Darren D. Sledjeski include Ohio University & University of Toledo.
Papers
More filters
Journal ArticleDOI
DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription
TL;DR: It is proposed that DsrA pairing stimulates RpoS translation by acting as an anti-antisense RNA, freeing the translation initiation region from the cis-acting antisense RNA and allowing increased translation.
Journal ArticleDOI
The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli.
TL;DR: While RpoS expression is very low in exponential phase at temperatures of 30 degrees C and above, at 20 degrees C there is substantial synthesis of RPOS during exponential growth, all dependent on DsrA RNA, and dsrA expression is also increased at low temperatures.
Journal ArticleDOI
Hfq Is Necessary for Regulation by the Untranslated RNA DsrA
TL;DR: It is shown that an hfq mutant strain is defective for DsrA-mediated regulation of both rpoS and hns, and Hfq acts as a protein cofactor for the regulatory activities of D srA by either altering the structure of DSRA or forming an active RNA-protein complex.
Journal ArticleDOI
Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAs
Peter J. Mikulecky,Meenakshi K Kaw,Cristin C. Brescia,Jennifer C. Takach,Darren D. Sledjeski,Andrew L. Feig +5 more
TL;DR: The bacterial Sm-like protein Hfq facilitates RNA-RNA interactions involved in post-transcriptional regulation of the stress response and is explained how it can simultaneously bind a ncRNA and its mRNA target to facilitate the strand displacement reaction required for H fq-dependent translational regulation.
Journal ArticleDOI
A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli.
TL;DR: It is found that the low level of expression from the rcsA promoter is due to transcriptional silencing by the histone-like protein H-NS; this silencing is sensitive to both sequence and context in a region upstream of the -35 region of the promoter.