D
David J. Guckenberger
Researcher at University of Wisconsin-Madison
Publications - 40
Citations - 1112
David J. Guckenberger is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Tumor progression & Substrate (printing). The author has an hindex of 12, co-authored 40 publications receiving 868 citations. Previous affiliations of David J. Guckenberger include Wisconsin Alumni Research Foundation.
Papers
More filters
Journal ArticleDOI
Micromilling: a method for ultra-rapid prototyping of plastic microfluidic devices
David J. Guckenberger,Theodorus E. de Groot,Alwin M. D. Wan,David J. Beebe,Edmond W. K. Young +4 more
TL;DR: This tutorial review offers protocols, tips, insight, and considerations for practitioners interested in using micromilling to create microfluidic devices to provide a potential user with information to guide them on whethermicromilling would fill a specific need within their overall fabrication strategy.
Journal ArticleDOI
Rapid Prototyping of Arrayed Microfluidic Systems in Polystyrene for Cell-Based Assays
Edmond W. K. Young,Erwin Berthier,David J. Guckenberger,Eric K. Sackmann,Casey Lamers,Ivar Meyvantsson,Anna Huttenlocher,David J. Beebe +7 more
TL;DR: A complete process is presented that describes the fabrication of polystyrene (PS) microfluidic devices with similar cost and time expenditures as PDMS-based devices with emphasis on creating methods that can compete with PDMS fabrication methods in terms of robustness, complexity, and time requirements.
Journal ArticleDOI
The VerIFAST: an integrated method for cell isolation and extracellular/intracellular staining
Benjamin P. Casavant,David J. Guckenberger,Scott M. Berry,Jacob T Tokar,Joshua M. Lang,David J. Beebe +5 more
TL;DR: The VerIFAST is demonstrated, a device that builds upon the simplified workflow of the Immiscible Filtration Assisted by Surface Tension to integrate a method for cellular isolation with methods for extra- and intracellular staining.
Journal ArticleDOI
Fundamentals of rapid injection molding for microfluidic cell-based assays.
Ulri N. Lee,Xiaojing Su,David J. Guckenberger,Ashley M. Dostie,Tianzi Zhang,Erwin Berthier,Ashleigh B. Theberge +6 more
TL;DR: Advantages and limitations of rapid injection molding for microfluidic device fabrication through measurement of key features for cell culture applications including channel geometry, feature consistency, floor thickness, and surface polishing are characterized.
Journal ArticleDOI
High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1
Jennifer L. Schehr,Zachery D. Schultz,Jay W. Warrick,David J. Guckenberger,Hannah M. Pezzi,Jamie M. Sperger,Erika Heninger,Anwaar Saeed,Ticiana Leal,Kara Mattox,Anne M. Traynor,Toby C. Campbell,Scott M. Berry,David J. Beebe,Joshua M. Lang +14 more
TL;DR: Interfering myeloid populations can be differentiated from true CTCs with additional staining criteria, thus improving the specificity of CTC identification and the accuracy of biomarker evaluation.