Dennis Huszar

TL;DR: Transgenic mice that express human IgM, IgG and Igκ in the absence of mouse IgM or Igκ are developed that contain human sequence transgenes that undergo V(D)J joining, heavy-chain class switching, and somatic mutation to generate a repertoire of human sequence immunoglobulins.

TL;DR: The upper limit of the cis-acting sequences necessary to direct heavy chain class switching and somatic mutation is defined - the sequences included within the transgene are sufficient to direct class switch recombination of the class-switched heavy chain genes.

TL;DR: Modified anti-dsDNA transgenic mice are described which allow us to study the site and developmental stage at which B-cell regulation occurs and show that in normal mice B cells expressing anti-DNA specificity are deleted in the bone marrow at a pre-B to immature B transitional stage.

TL;DR: It is shown here that immunization of JHD mice with soluble antigen fails to prime CD4 T cells, for either clonal expansion or delivery of immunological help for antibody responses, demonstrating that B cells play a critical role inCD4 T cell priming, possibly by delivering a critical co-stimulatory activity forClonal expansion of CD 4 T cells.

TL;DR: The induction of a novel ribonucleotide reductase after herpes simplex virus infection of mammalian cells is confirmed and the enzyme induced was much more labile than the control enzyme upon purification.