D
Detlev Riesner
Researcher at University of Düsseldorf
Publications - 210
Citations - 9640
Detlev Riesner is an academic researcher from University of Düsseldorf. The author has contributed to research in topics: Viroid & Potato spindle tuber viroid. The author has an hindex of 49, co-authored 209 publications receiving 8945 citations. Previous affiliations of Detlev Riesner include Forschungszentrum Jülich.
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Journal ArticleDOI
Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures.
TL;DR: Viroids are uncoated infectious RNA molecules pathogenic to certain higher plants and exhibit high thermal stability, cooperativity, and self-complementarity resulting in a rod-like native structure.
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Synthetic Mammalian Prions
Giuseppe Legname,Ilia V. Baskakov,Hoang-Oanh B. Nguyen,Detlev Riesner,Fred E. Cohen,Stephen J. DeArmond,Stanley B. Prusiner +6 more
TL;DR: The results provide compelling evidence that prions are infectious proteins and suggest that a novel prion strain was created.
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Temperature-gradient gel electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts.
TL;DR: It could be shown that by this method dsRNA molecules may be differentiated which differ only in one base-pair, or proteins differing in one amino acid only, as a particular advantage, temperature-gradient gel electrophoresis allows the study of conformational transitions of biopolymers which have not been purified.
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Prion detection by an amyloid seeding assay.
David W. Colby,Qiang Zhang,Shuyi Wang,Darlene Groth,Giuseppe Legname,Detlev Riesner,Stanley B. Prusiner +6 more
TL;DR: The authors' amyloid seeding assay (ASA) detected PrPSc, the sole component of the prion, in brain samples from humans with sporadic Creutzfeldt–Jakob disease, as well as in rodents with experimental prion disease.
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Temperature-gradient gel electrophoresis of nucleic acids: analysis of conformational transitions, sequence variations, and protein-nucleic acid interactions.
Detlev Riesner,Gerhard Steger,Rolf Zimmat,Robert A. Owens,Manfred Wagenhöfer,Wolfgang Hillen,Silke Vollbach,Karsten Henco +7 more
TL;DR: Temperature‐gradient gel electrophoresis (TGGE) is applied to analyze conformational transitions and sequence variations of nucleic acids and protein‐nucleic acid interactions and the results are discussed under the aspect that TGGE may be applied as routine analytical laboratory procedure.