Showing papers by "Dharam P. Agarwal published in 1986"
TL;DR: A rare case of human liver cytosolic aldehyde dehydrogenase (isozyme II) variation discovered in a Chinese autopsy liver specimen is reported, and the existence of additional minor bands indicates the presence of tetramer hybrid forms made up of normal and variant monomers.
Abstract: A rare case of human liver cytosolic aldehyde dehydrogenase (isozyme II) variation discovered in a Chinese autopsy liver specimen is reported. While the major isozyme band was nearly absent, several additional minor bands were observed on isoelectric focusing gel. Rabbit antibodies to purified human liver ALDH II showed immunological cross-reactivity for the variant enzyme bands. The existence of additional minor bands indicates the presence of tetramer hybrid forms made up of normal and variant monomers. The observed abnormality may represent the heterozygous form of ALDH II variation. A similar variant was also detected in erythrocytes of a male Thai student.
25 citations
TL;DR: Southern blot analysis of human-rodent somatic cell hybrids indicates that the human ALDHI gene resides on chromosome 12, and a cloned 850 bp cDNA fragment corresponding to the 3′-coding part of humanALDHI-mRNA was used as a probe for the chromosomal assignment of the ALD HI gene.
Abstract: A cloned 850 bp cDNA fragment corresponding to the 3'-coding part of human ALDHI-mRNA was used as a probe for the chromosomal assignment of the ALDHI gene. Southern blot analysis of human-rodent somatic cell hybrids indicates that the human ALDHI gene resides on chromosome 12.
15 citations
TL;DR: Findings suggest a slower conversion of ethanol to carbon dioxide in aldehyde dehydrogenase deficient individuals, which may be another consequence of this deficiency besides the higher plasma acetaldehyde levels observed after ethanol loading in comparison to individuals with normal aldechemical activity.
5 citations
TL;DR: Human fetal muscle cDNA library was screened with aβ-myosin heavy chain gene fragment containing Alu sequences and two cDNA clones AI and BII with 1.8 and 3 kb inserts respectively, which appeared to contain repetitive sequences as well as single copy regions.
Abstract: Human fetal muscle cDNA library was screened with aβ-myosin heavy chain gene fragment containing Alu sequences. Two cDNA clones AI and BII with 1.8 and 3 kb inserts respectively were chosen for further characterization by means of RNA and DNA hybridization procedures and sequencing. The clones appeared to contain repetitive sequences as well as single copy regions. They are actively transcribed in different stages of myogenic development but not in the liver. DNA sequence analysis of short stretches from both clones revealed no sequence homology to any other published DNA sequences.
2 citations