Dieter Cech

TL;DR: Conjugates with PEG coupled to both 3'- and 5'-terminal positions showed a more than 10-fold increase in exonuclease stability, and conjugation had little effect on the hybridization behavior of oligonucleotide conjugates with unmodified complementary oligon DNA strands.

TL;DR: (4, 4′-Dimethoxytrityl)-polyethylene glycol-(2-cyanoethyl-N, N-diisopropyl)-phosphoramidites and (4, 5′-dimethoxy trityl) glycol derivatized glass supports were synthesized and used to introduce PEG with polymerization degrees from about 6 to more than 100 at 3′- and 5′

TL;DR: It is shown that endonucleolysis of the stimulator molecules is not a necessary prerequisite of enzyme activation, and data are in agreement with a model whereby EcoRII simultaneously interacts with two recognition sites in the active complex, but cleavage of the site serving as an allosteric activator is not necessary.

TL;DR: Of a series of 5-substituted 1-beta-D-arabinofuranosyluracil analogues, (E)-5-(2-bromovinyl)-araU(BrVaraU) and 5-vinyl-araU (Vara U) were the most potent inhibitors of plaque formation by two herpes simplex virus type 1 (HSV-1) strains in human embryonic lung fibroblast (HELF) cell cultures.

TL;DR: Chemical synthesis of oligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are described and the methylmercapto function of 2 is replaced by an amino group in the final ammonia treatment used for cleavage from support and base deprotection.