Developments in industrially important thermostable enzymes: a review.

A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing

One stop mycology

https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2672.1995.tb03102.x

The application of magnetic separations in applied microbiology

Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1: Characterization and potential application as detergent additive

Purification and characterization of a thermostable alpha-amylase from Bacillus licheniformis

This paper is focused on the possibility to apply the magnetic stabilization technique in bioprocessing. The feasibility of a continuous ethanol fermentation process with immobilizedSaccharomyces cerevisiae cells in a magnetically stabilized bed (MSB) was demonstrated. The fermentation processes were carried out in an external magnetic field, transverse to the fluid flow. The flexibility to change the bed expansion owing to the independent change of the fluid flow and the field intensity (the “magnetization FIRST” mode) permitted the creation of fixed beds with different particle arrangements, which affected the bed porosity, the effective fluid-particle contact area, and the mass transfer processes on the particle-fluid interface. As a result, higher ethanol concentration, ethanol production, and glucose uptake rates than in conventional packed bed reactor were reached.

Performance of a magnetically stabilized bed reactor with immobilized yeast cells.

Non-porous magnetic supports for cell immobilization

Cells of the moderately thermophilic Bacillus sp. UG-5B strain, producing nitrilase (EC3.5.5.1), which converts nitriles directly to the corresponding acid and ammonia, were immobilized using different types of matrices and techniques. A variety of sol-gel silica hybrids were tested for entrapment and adsorption of bacterial cells as well as chemical binding on polysulphone membranes. Activation of the matrix surface with formaldehyde led to an increase in immobilization efficiency and operational stability of the biocatalysts. Among the supports screened, membranes gave the best results for enzyme activity and especially operational stability, with retention of 100% activity after eight reaction cycles.

E. Dobreva

Papers

Purification and characterization of a thermostable alpha-amylase from Bacillus licheniformis

Performance of a magnetically stabilized bed reactor with immobilized yeast cells.

Non-porous magnetic supports for cell immobilization

Immobilization of cells with nitrilase activity from a thermophilic bacterial strain.

Influence of the immobilization conditions on the efficiency of α-amylase production by Bacillus licheniformis