Elena Mori

TL;DR: The randomly amplified polymorphic DNA (RAPD) fingerprinting technique was applied to a collection of 101 strains of the genus Bacillus, including 61 strain of the B. cereus group and identified an 838-bp RAPD marker specific for Bacillus anthracis.

TL;DR: The available evidence supports the hypothesis that histidine biosynthesis was assembled by a gene recruitment process and suggests that it is a highly conserved pathway that was probably already present in the last common ancestor of all extant life.

TL;DR: It is concluded that RAPD fingerprinting, combined with the analysis of RFLP, can provide unambiguous type assignment in yeasts and is a powerful tool for yeast differentiation and identification.

TL;DR: The structure of the yeast HIS6 gene supports the two-step evolutionary model and suggests that these two successive paralogous gene duplications took probably place in the early steps of molecular evolution of the histidine pathway, well before the diversification of the three domains, and that this pathway was one of the metabolic activities of the last common ancestor.

TL;DR: The complexity of the annealing process suggested that, in the studied reaction conditions, many primer-templateAnnealing sites were extended in the first cycles and that differences in the efficiency of priming and replication processes led to amplification of RAPD fragments.