E
Elsie M. Williams
Researcher at Victoria University of Wellington
Publications - 26
Citations - 464
Elsie M. Williams is an academic researcher from Victoria University of Wellington. The author has contributed to research in topics: Nitroreductase & Prodrug. The author has an hindex of 9, co-authored 23 publications receiving 332 citations. Previous affiliations of Elsie M. Williams include Emory University & Wellington Management Company.
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Journal ArticleDOI
Nitroreductase gene-directed enzyme prodrug therapy: insights and advances toward clinical utility
Elsie M. Williams,Rory F Little,Alexandra M. Mowday,Michelle H. Rich,Jasmine V.E. Chan-Hyams,Janine N. Copp,Jeff B. Smaill,Adam V. Patterson,David F. Ackerley,David F. Ackerley +9 more
TL;DR: This review examines the vast catalytic and therapeutic potential offered by type I nitroreductase enzymes in partnership with nitroaromatic prodrugs, with particular focus on gene-directed enzyme prodrug therapy (GDEPT; a form of cancer gene therapy).
Journal ArticleDOI
Engineering a Multifunctional Nitroreductase for Improved Activation of Prodrugs and PET Probes for Cancer Gene Therapy.
Janine N. Copp,Alexandra M. Mowday,Elsie M. Williams,Christopher P. Guise,Amir Ashoorzadeh,Abigail V. Sharrock,Jack U. Flanagan,Jeff B. Smaill,Adam V. Patterson,David F. Ackerley,David F. Ackerley +10 more
TL;DR: Evidence is provided that the dominant contribution to improved PR-104A activity was enhanced affinity for the prodrug over-competing intracellular substrates, suggesting optimal usefulness in bacterial rather than viral GDEPT vectors.
Journal ArticleDOI
Creation and screening of a multi-family bacterial oxidoreductase library to discover novel nitroreductases that efficiently activate the bioreductive prodrugs CB1954 and PR-104A
Gareth A. Prosser,Janine N. Copp,Alexandra M. Mowday,Christopher P. Guise,Sophie P. Syddall,Elsie M. Williams,Claire N. Horvat,Pearl M. Swe,Amir Ashoorzadeh,William A. Denny,Jeff B. Smaill,Adam V. Patterson,David F. Ackerley,David F. Ackerley +13 more
TL;DR: The utility of a flexible screening system based on the Escherichia coli SOS response to evaluate novel nitroreductase enzymes and prodrugs in concert is demonstrated, and a library of 47 candidate genes representing 11 different oxidoreductase families was created and screened to identify the most efficient activators of two different nitroaromatic pro drugs.
Journal ArticleDOI
Pore Engineering for Enhanced Mass Transport in Encapsulin Nanocompartments.
TL;DR: This study demonstrates the high tolerance of encapsulin for protein engineering and has created a set of novel, functionally improved scaffolds for applications as bionanoreactors.
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Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity.
TL;DR: Evidence is presented that the reduction of quinones by NfsA is most consistent with a single-step (H-) hydride transfer mechanism, and this step is much faster than the steady-state turnover number.