E
Emilio Alvarez
Researcher at University of León
Publications - 21
Citations - 1069
Emilio Alvarez is an academic researcher from University of León. The author has contributed to research in topics: Penicillium chrysogenum & Acyltransferase. The author has an hindex of 14, co-authored 21 publications receiving 1037 citations.
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Journal ArticleDOI
High–Frequency Transformation of Penicillium Chrysogenum
TL;DR: A transformant strain showed a fivefold higher orotidine–5′–monophosphate decarboxylase activity than the wild type strain, indicating that the genes carried in the transforming vector are efficiently expressed in Penicillium.
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Cloning and characterization of the acyl-coenzyme A: 6-aminopenicillanic-aid-acyltransferase gene of Penicillium chrysogenum
J. L. Barredo,P. van Solingen,Bruno Díez,Emilio Alvarez,Jesús M. Cantoral,A. Kattevilder,E.B. Smaal,Martinus Antonius Math Groenen,Annemarie Eveline Veenstra,Juan F. Martín +9 more
TL;DR: The aat gene of P. chrysogenum resembles the genes encoding Pn acylases of Escherichia coli, Proteus rettgeri and Pseudomonas sp.
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Purification to homogeneity and characterization of acyl coenzyme A:6-aminopenicillanic acid acyltransferase of Penicillium chrysogenum.
TL;DR: The acyl coenzyme A (CoA):6-aminopenicillanic acid (6-APA) acyltransferase of Penicillium chrysogenum AS-P-78 was purified to homogeneity, as concluded by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing.
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Large amplification of a 35-kb DNA fragment carrying two penicillin biosynthetic genes in high penicillin producing strains of Penicillium chrysogenum.
TL;DR: In this article, the isopenicillin N synthase (pcbC) and acyl-CoA:6-APA acyltransferase (penDE) genes of Penicillium chrysogenum were located in a 19.5-kb DNA fragment that had been previously cloned in phage vector EMBL3.
Journal ArticleDOI
The isopenicillin-N acyltransferase of Penicillium chrysogenum has isopenicillin-N amidohydrolase, 6-aminopenicillanic acid acyltransferase and penicillin amidase activities, all of which are encoded by the single penDE gene
Emilio Alvarez,Boudewijn Meesschaert,Eduardo Montenegro,Santiago Gutiérrez,Bruno Díez,José Luis Barredo,Juan F. Martín +6 more
TL;DR: An amino acid sequence similar to that of the active site of thioesterases was found in the isopenicillin-N acyltransferase, suggesting that this site is involved in the transfer of phenylacetyl residues from phenyl acetyl thioesters.