Showing papers by "Enzo A. Palombo published in 1998"
TL;DR: Severe rotav virus disease in young children may be followed by extended excretion of rotavirus, which may be greater than previously believed and explain some cases of the postgastroenteritis syndrome.
137 citations
TL;DR: Development of a simplified culture method for astrov virus positive faecal specimens improved the sensitivity of astrovirus-specific RT-PCR from 52 to 100% and should be of value as a confirmatory test in surveys of human astroviral infection.
32 citations
Journal Article•
TL;DR: An atypical human rotavirus strain Z10262, isolated from a chronically infected immunodeficient child, displayed an unusual genomic RNA electrophoretic pattern and was derived from a genetic rearrangement which involved a partial duplication of the open reading frame encoding the non-structural protein NSP5.
Abstract: An atypical human rotavirus strain Z10262, isolated from a chronically infected immunodeficient child, displayed an unusual genomic RNA electrophoretic pattern. Besides, Northern blot analysis indicated that this strain contained an abnormally migrating gene 11 equivalent. Sequencing of this gene showed that it was derived from a genetic rearrangement which involved a partial duplication of the open reading frame (ORF) encoding the non-structural protein NSP5. However, the duplicated region contained a deletion and several point mutations relative to the first copy of the ORF. Phylogenetic analysis of human and animal NSP5 amino acid sequences including Z10262 revealed two groups of human proteins related to different animal proteins. The isolation and analysis of Z10262 strain provides further evidence for the genetic complexity of naturally occurring human rotaviruses.
15 citations
TL;DR: Comparisons of the neutralization epitope regions of VP7 indicated that most strains might exhibit antigenic differences to DS1, which is distantly related to strain DS1 whose VP7 is included in the rhesus rotavirus tetravalent vaccine formulation.
Abstract: The sequences of the genes encoding the outer cap sid glycoprotein, VW, of twelve serotype G2 rotavi-rus isolates from Australia were determined. The deduced amino acid sequences were compared by phylogenetic analysis to published sequences from strains collected from various geographical locations worldwide between 1976 an 1993. This analysis showed that geographical clustering of strains was apparent and that most strains were distantly related to strain DS1 whose VP7 is included in the rhesus rotavirus tetravdent vaccine formulation. Comparisons of the neutralization epitope regions of VP7 also indicated that most strains might exhibit anti-genic differences to DSI. These results have implications for the development of future vaccine strategies.
13 citations