Showing papers by "Farzad Kobarfard published in 2004"
TL;DR: The organism grew better and culture extract showed higher level of antifungal activity when malt extract plus glucose were used as carbon source and yeast extract was used as nitrogen source and the lowest level of growth and antifundal activity were observed when starch was usedAs carbon source for growth of the fungus.
Abstract: Growth and production of antifungal agent by Mycena leptocephala was investigated in different culture media composition at various initial pH and temperatures. Maximum growth and activity was observed at the initial pH of 5.5 and 25oC. No detectable growth and activity was observed at pH of 3.5 and 7.5. Growth of the fungus and antifungal activity were also very low at 37 o C and 20 o C. The organism grew better and culture extract showed higher level of antifungal activity when malt extract plus glucose were used as carbon source and yeast extract was used as nitrogen source. Meanwhile the lowest level of growth and antifungal activity were observed when starch was used as carbon source for growth of the fungus. Key Words: Culture conditions, basidiomycetes, bioactive compounds, carbon source, nitrogen source and temperature. African Journal of Biotechnology Vol.3(11) 2004: 606-609
49 citations
01 Jan 2004
TL;DR: Two isoflavone, irilin A and irisone B were isolated from Iris songarica and their structures were determined by 1 and 2 D-NMR, IR, UV and MS as mentioned in this paper.
Abstract: Two isoflavone, irilin A and irisone B were isolated from Iris songarica and their structures were determined by 1 and 2 D-NMR, IR, UV and MS. The effect of solvent on 1 H-NMR spectra of irisone B have been studied.
8 citations
TL;DR: Under aerobic conditions, phenylenediamine induced cytotoxicity and ROS formation were markedly increased by inactivating DT-diaphorase but were prevented by a subtoxic concentration of the mitochondrial respiratory inhibitor cyanide, which suggests that the H 2 O 2 generation could be attributed to a futile two electron redox cycle.
Abstract: It has already been reported that muscle necrosis induced by various phenylenediamine derivatives are correlated with their autoxidation rate Now in a more detailed investigation of the cytotoxic mechanism using a model system of isolated hepatocytes and ring-methylated structural isomer durenediamine (DD) we have shown that under aerobic conditions, phenylenediamine induced cytotoxicity and ROS formation were markedly increased by inactivating DT-diaphorase but were prevented by a subtoxic concentration of the mitochondrial respiratory inhibitor cyanide This suggests that the H 2 O 2 generation could be attributed to a futile two electron redox cycle involving oxidation of phenylenediamine to the corresponding diimine by the mitochondrial electron transfer chain and re-reduction by the DT- diaphorase The subcellular organelle oxidative stress effects leading to cytotoxicity has not yet been identified Hepatocyte mitochondrial membrane potential was only slightly decreased by phenylenediamine before cytotoxicity ensued However phenylenediamine induced lysosomal damage and hepatocyte protease activation Endocytosis inhibitors, lysosomotropic agents or lysosomal protease inhibitors also prevented phenylenediamine induced cytotoxicity Furthermore desferoxamine (a ferric chelator), antioxidants or ROS scavengers (catalase, mannitol, tempol or dimethylsulfoxide) prevented phenylenediamine cytotoxicity It is concluded that H 2 O 2 reacts with lysosomal Fe 2+ to form “ROS” which causes lysosomal lipid peroxidation, membrane disruption, protease release and cell death
4 citations
3 citations