Feipeng Philip Yu

TL;DR: Epifluorescence micrographs of frozen biofilm cross sections clearly revealed gradients of respiratory activity within biofilms in response to monochloramine treatment, quantified by calculating the ratio of CTC and DAPI intensities measured by image analysis.

TL;DR: This minireview focuses on the application of fluorogenic compounds in the detection of bacteria with particular emphasis on the assessment of physiological activity using epifluorescence microscopy.

TL;DR: Viable cell densities within biofilms determined by the three in situ methods were comparable and always showed approximately 2-fold higher values than those obtained with the PC method.

TL;DR: In situ enumeration methods using fluorescent probes and a radioisotope labelling technique were applied to evaluate physiological changes of Klebsiella pneumoniae within biofilms after disinfection treatment, showing a range of responses and suggested different physiological reactions inBiofilms exposed to chlorine and monochloramine.

TL;DR: In this biofilm system, measures of microbial respiratory activity and culturability yielded widely differing estimates of biocide efficacy, and integrated measures of biofilm respiratory activity, including net oxygen and glucose utilization rates, showed only a 10 to 15% reduction.