Showing papers by "Fernando Ferreira published in 2002"

Sorovares de leptospiras predominantes em exames sorológicos de bubalinos, ovinos, caprinos, eqüinos, suínos e cães de diversos estados brasileiros

Abstract: From 1984 to 1997 15.558 sorologic tests to leptospirosis were performed (MAT with 24 leptospires serovars) in 284 sheeps, 879 boffaloes, 983 dogs, 1.941 goats, 2.903 horses and 8.568 swines. The distribuition of animals examined by species and state were: sheeps - SP (100%); buffaloes - SP (100%); dogs - SP (80.7%), RS (0.10%), SC (0.10%) and PI (19.0%); goats - SP (33.1%), PB (63.7%) and CE (3.2%); horses - SP (79.3%), RS (9.98%), SC (0.62%), PR (2.5%), RJ (0.17%), MG (1.96%), MT (3.99%), PB (1.3%) and PI (0.03%); swines - SP (61.91%), RS (0.3%), SC (5.95%), PR (3.67%), RJ (0.88%), MG (24.38%), GO (1.12%), SE (0.2%), PE (0.90%), CE (0.34%) and MA (0.1%). From the sheeps tested, 54.5% were examined between 1996 and 97. 33.3% between 1989 and 1990 and 12.2% in the other years; buffaloes - 21.7% from 1984 to 95 and 78.83% between 1996 and 97; dogs - 16.91% from 1984 to 92 and 83.09% from 1993 to 97; goats - 6.97% from 1984 to 91 and 93.09% from 1992 to 97, althought 49% were concerning to 1992; horses - 18.1% from 84 to 90 and 81.9% from 1991 to 97; swines - 61.16% concerning 1990, 91, 95 and 96. The proportion of reactors to at least one leptospira serovar presented the following average and most frequent serovars: sheeps - 0.70% and icterohaemorrhagiae like most frequent serovar; buffaloes - 43.7% and serovars hardjo and pomona; dogs - 17.7% and serovar icterohaemorrhagiae in SP and pyrogenes in PI; goats - 4.17% and icterohaemorrhagiae and grippotyphosa in CE, icterohaemorrhagiae in PB and pyrogenes in SP; horses - 29% and serovar icterohaemorrhagiae in PR, SC, SP, RJ e MG, grippotyphosa in MT, pyrogenes in PB and patoc in RS; swines -24.46% and serovar grippotyphosa and icterohaemorrhagiae in MG, pomona in RS, pomona and icterohaemorrhagiae in PE and RJ, autumnalis in CE and icterohaemorrhagiae in GO, PR, SC e SP.

Prevalence of Neospora caninum Antibodies in Dogs From Dairy Cattle Farms in Parana, Brazil

Abstract: Serum samples from 134 dogs from 22 cattle dairy farms in the northern region of Parana State, Brazil, were tested for antibodies to Neospora caninumin an indirect fluorescent antibody test Antibodies ($1:50) to N caninum were found in 29 (216%) of the 134 dogs, and seropositive dogs were found on 14 (636%) of the 22 dairy cattle farms The antibody titers of dogs were 1:50 (3 dogs), 1:100 (7 dogs), 1:200 (7 dogs), 1:400 (6 dogs), and $1:800 (6 dogs) The low prevalence (9%) in ,1-yr-old dogs compared with the 2- to 3-fold higher preva- lence in older dogs (17-29%) suggests postnatal exposure to N caninum infection

Inquérito sorológico para leptospirose em cães do município de santana de parnaíba, são paulo, utilizando a campanha de vacinação anti-rábica do ano de 1999

Abstract: This work aimed to evaluate the zoonotic potential of the canine population in the City of Santana de Parnaiba, State of Sao Paulo, related to leptospirosis. This study was held during the 1999 rabies vaccination campaign, when 410 serum samples were collected and a questionnaire was applied to the dogs’ owners to describe an epidemiologic profile of this population and conduce the statistical analysis, to assess the risk factors. The diagnostic method run for leptospiroses was the microtechnique of microscopic agglutination using a batch of 22 leptospiral serovars. The results obtained indicated 15% positive serum samples and the most frequent reactant serovars were copenhageni (24%), canicola (16%) and hardjo (16%). The statistical analysis of the questionnaire showed significant correlation between animal age and positive serum samples for leptospirosis.

Soroprevalência da anemia infecciosa eqüina, da arterite viral dos eqüinos e do aborto viral eqüino no município de Uruará, PA, Brasil

Abstract: Equine infection anemia virus (EIAV), Equine arteritis virus (EAV) and Equine herpesvirus 1 (EHV 1) are the causal agents of diseases which may bring economical losses. The aim o of this study was to estimate the prevalence of herds and animals infected with EIA, EAV and EHV in Uruara municipal district, Para State-Brazil. Antibodies against EIAV were detected by the immunodiffusion test and those against EAV and HEV-1 by the serum neutralization test. Sample size was estimated from 2069 holder farms that raised Equidae and did not vaccinate against EAV and EHV. A 90% confidence level was adopted with 15% precision and 50% estimated prevalence. The herd was considered positive when it had at least one positive animal. The following prevalence of serum reactors animals were observed: VAIE: 17,71% (IC 10,67 - 26,83%), HVE-1: 17,71% (IC 10,67 - 26,83%) and VAVE: 0,00% (IC 0,00 - 3,77%). The following prevalence of positive herds were observed: VAIE: 53% (IC 38,12 - 68,12%), HVE-1: 40.62% (IC 25.96 - 56.65%) and VAVE: 0% (IC 0 - 6.94%).

Determinação da contagem de ovos de nematódeos no período peri-parto em vacas

Abstract: Dois experimentos foram conduzidos com o objetivo de se observar as alteracoes do numero de ovos de nematodeos por grama de fezes (OPG) eliminados por vacas no periodo do peri-parto No primeiro foram utilizadas vacas de racas europeia (leiteiras) e zebuinas (de corte), da 6a semana pre-parto a 6a semana pos-parto Observou-se diferenca significativa (p<0,05) com maior numero de animais leiteiros apresentando ovos de nematodeos nas fezes durante o peri-parto Na categoria de animais leiteiros, houve um aumento significativo no OPG no momento do parto e na semana quatro pos-parto, em relacao ao pre-parto Tambem nesta categoria, observou-se uma associacao negativa entre o numero de partos e a positividade em relacao ao OPG (p<0,05) Devido ao comportamento diferenciado dos animais leiteiros um segundo experimento foi feito para se comparar, nessa categoria animal, as alteracoes de OPG da quarta semana pre-parto a decima pos-parto, em vacas de primeira e segunda crias e tres ou mais crias Observou-se diferenca significativa, com os animais de primeira e segunda crias apresentando maiores valores de OPG nas semanas dois, tres e quatro pos-parto (p<0,05) Os valores de OPG para os animais de primeira e segunda crias foram de 0 - 1800, enquanto nos animais mais velhos a variacao foi de 0 - 150 OPG Os resultados de ambos os estudos sugerem que um esquema de controle contra nematodeos gastrintestinais deva ser implantado em vacas de primeira e segunda crias com o uso de anti-helminticos no pre-parto, ao redor da segunda semana antes dos nascimentos

Estudo comparativo de dois métodos de descontaminação na pesquisa de micobactérias

Abstract: The present work had for objective compare the performance of two methods of decontamination in mycobacteria isolation from feces. One of the protocols used the method of Petroff - basic method, using the solution of sodium hidroxid (NaOH) 4%, the other used Lowenstein-Jensen modified method - acid method with sulfuric acid solution (H2SO4) 4%. Thirty females hamsters were inoculated orally with 108 cfu/animal of Mycobacterium avium and your feces were examinated 192 differents experimental moments. Each sample was submitted to the basic and acid method. Mycobacteria was isolated in 62 (32.3%) chances in the basic method and in 56 (29.2%) in the acid method. The “kappa” value was 0.6 demonstrating moderate agreement between them. The Petroff method can be utilized for the decontamination of feces and it is important to test others concentrations than 4% of the sulfuric acid solution in the fetching of the maximum decontamination of the sample and minor delay of the viability and capacity of multiplication of the mycobacteria.

Soroprevalência de anticorpos anti-rotavírus do sorogrupo A em bovinos de uma fronteira pioneira na Amazônia brasileira

Abstract: Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruara municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group.

Avaliação da virulência em hamsters (Mesocricetus auratus) de estirpes de Mycobacterium avium presentes na população de suínos do sul do Brasil

Abstract: The finding of four clusters of M. avium (PIG-A, B, C and D), typed by the IS1245-RFLP method, infecting the swine population of the south region of Brazil, the possible existence of virulence differences among them, the role of the virulence in the transmission mechanisms of infections and the existence of reasonable doubts regarding the importance of horizontal transmission for swine micobacteriosis, the virulence of these four strains of M. avium were compared. Bacteria from each cluster were inoculated in 48 hamsters by intra-peritoneal route. On the 2nd, 13th, 26th, and 40th days after inoculation, (T1 to T4), 12 animals of each cluster were sacrificed with vapors of ethyl ether and the bacteria were quantified in the liver, spleen and lung. Results were expressed as cfu/g of organ. The presence of the strains was verified in the blood and histological exams were also accomplished. The four strains induced granulomatous lesions in the liver and spleen since 2 days after inoculation and were disseminated to the lungs through the blood stream. The cfu counts from spleen were always bigger them that obtained from liver and lungs. Differences among strains were observed through the analysis of cfu counts from spleen (T1: p PIG-A> PIG-D> PIG-C.