Showing papers by "Francis V. Chisari published in 1978"

Regulation of Human Lymphocyte Function by a Soluble Extract from Normal Human Liver

Abstract: The blastogenic and DNA synthetic response of human peripheral blood lymphocytes (PBL) to phytohemagglutinin (PHA) and allogeneic cells can be inhibited by a nontoxic aqueous extract (LEx) of normal human liver LEx reversibly inhibits the activation of PBL by PHA, arrests ongoing DNA synthesis, and limits the duration of the DNA synthetic response to PHA at concentrations as low as 07 to 15 µg LEx protein/culture In contrast, human T lymphocyte E rosette formation is unaffected by LEx concentrations in excess of 900 µg/culture LEx has been partially purified by ultracentrifugation, ammonium sulfate precipitation, and molecular exclusion chromatography and appears to be a heat labile protein with a mw of approximately 65,000 and an isoelectric point of approximately 408 LEx is distinct from other previously described human immunoregulatory molecules and is potentially releasable in vivo from injured or necrotic liver cells Because of its potency and anatomic distribution LEx may potentially modulate immunopathogenetic events responsible for assorted inflammatory and neoplastic liver diseases