In addition to the often-cited advantages of using Arabidopsis thaliana as a model system in plant biological research (1), Arabidopsis has many additional characteristics that make it an attractive experimental organism for studying lea d (Pb) accumulation and tolerance in plants. These include its fortuitous familial relationship to many known metal hyperaccumulators (Brassicaceae), as well as similar Pbaccumulation patterns to most other plants. Using nutrient-agar plates, hydroponic culture, and Pb-contaminated soils as growth media, we found significant variation in Arabidopsis thaliana ecotypes in accumulation and tolerance of Pb. In addition, we have found that Pb accumulation is not obligatorily linked with Pb tolerance, suggesti ng that different genetic factors control these two processes. We also screened ethyl methanesulfonate-mutagenized M2 populations and identified several Pb-accumulating mutants. Current characterization of these mutants indicates that their phenotypes are likely due to alteration of general metal ion uptake or translocation processes since these mutants also accumulate many other metals in shoots. We expect that further characterization of the ecotypes and mutants will shed light on the basic genetic and physiological underpinnings of plant-based Pb remediation. 7. Aromatic nitroreduction of acifluorfen in soils, rhizospheres, and pure cultures of rhizobacteria. Zablotowicz, R. M., Locke, M. A., and Hoagland, R. E. Phytoremediation of soil and water contaminants. Washington, DC : American Chemical Society, 1997. p. 38-53. NAL Call #: QD1.A45-no.664 Abstract: Reduction of nitroaromatic compounds to their corresponding amino derivatives is one of several pathways in the degradation of nitroxenobiotics. Our studies with the nitrodiphenyl ether herbicide acifluorfen showed rapid metabolism to am inoacifluorfen followed by incorporation into unextractable soil components in both soil and rhizosphere suspensions. Aminoacifluorfen was formed more rapidly in rhizospheres compared to soil, which can be attributed to higher microbial populations, espec ially of Gram-negative bacteria. We identified several strains of Pseudomonas fluorescens that possess nitroreductase activity capable of converting acifluorfen to aminoacifluorfen. Factors affecting acifluorfen nitroreductase activity in pure cultures an d cell-free extracts, and other catabolic transformations of acifluorfen, ether bond cleavage, are discussed. Plant rhizospheres should be conducive for aromatic nitroreduction. Nitroreduction by rhizobacteria is an important catabolic pathway for the ini tial degradation of various nitroherbicides and other nitroaromatic compounds in soils under Reduction of nitroaromatic compounds to their corresponding amino derivatives is one of several pathways in the degradation of nitroxenobiotics. Our studies with the nitrodiphenyl ether herbicide acifluorfen showed rapid metabolism to am inoacifluorfen followed by incorporation into unextractable soil components in both soil and rhizosphere suspensions. Aminoacifluorfen was formed more rapidly in rhizospheres compared to soil, which can be attributed to higher microbial populations, espec ially of Gram-negative bacteria. We identified several strains of Pseudomonas fluorescens that possess nitroreductase activity capable of converting acifluorfen to aminoacifluorfen. Factors affecting acifluorfen nitroreductase activity in pure cultures an d cell-free extracts, and other catabolic transformations of acifluorfen, ether bond cleavage, are discussed. Plant rhizospheres should be conducive for aromatic nitroreduction. Nitroreduction by rhizobacteria is an important catabolic pathway for the ini tial degradation of various nitroherbicides and other nitroaromatic compounds in soils under phytoremediation management. 8. Ascorbate: a biomarker of herbicide stress in wetland plants. Lytle, T. F. and Lytle, J. S. Phytoremediation of soil and water contaminants. Washington, DC : American Chemical Society, 1997. p. 106-113. NAL Call #: QD1.A45-no.664 Abstract: In laboratory exposures of wetland plants to low herbicide levels (<0.1 micrograms/mL), some plants showed increased total ascorbic acid suggesting a stimulatory effect on ascorbic acid synthesis occurred; at higher herbicide conce ntrations (greater than or equal to 0.1 micrograms/mL) a notable decline in total ascorbic acid and increase in the oxidized form, dehydroascorbic acid occurred. Vigna luteola and Sesbania vesicaria were exposed for 7 and 21 days respectively to atrazine (0.05 to 1 microgram/mL); Spartina alterniflora 28 days at 0.1 micrograms/mL trifluralin; Hibiscus moscheutos 14 days at 0.1 and 1 microgram/mL metolachlor in fresh and brackish water. The greatest increase following low dosage occurred with S. alterniflo ra, increasing from <600 micrograms/g wet wt. total ascorbic acid to >1000 micrograms/g. Ascorbic acid may be a promising biomarker of estuarine plants exposed to herbicide runoff; stimulation of ascorbic acid synthesis may enable some wetland plant s used in phytoremediation to cope with low levels of these compounds. In laboratory exposures of wetland plants to low herbicide levels (<0.1 micrograms/mL), some plants showed increased total ascorbic acid suggesting a stimulatory effect on ascorbic acid synthesis occurred; at higher herbicide conce ntrations (greater than or equal to 0.1 micrograms/mL) a notable decline in total ascorbic acid and increase in the oxidized form, dehydroascorbic acid occurred. Vigna luteola and Sesbania vesicaria were exposed for 7 and 21 days respectively to atrazine (0.05 to 1 microgram/mL); Spartina alterniflora 28 days at 0.1 micrograms/mL trifluralin; Hibiscus moscheutos 14 days at 0.1 and 1 microgram/mL metolachlor in fresh and brackish water. The greatest increase following low dosage occurred with S. alterniflo ra, increasing from <600 micrograms/g wet wt. total ascorbic acid to >1000 micrograms/g. Ascorbic acid may be a promising biomarker of estuarine plants exposed to herbicide runoff; stimulation of ascorbic acid synthesis may enable some wetland plant s used in phytoremediation to cope with low levels of these compounds. 9. Atmospheric nitrogenous compounds and ozone--is NO(x) fixation by plants a possible solution. Wellburn, A. R. New phytol. 139: 1 pp. 5-9. (May 1998). NAL Call #: 450-N42 Descriptors: ozoneair-pollution nitrogen-dioxide nitric-oxide air-quality tolerancebioremediationacclimatizationnutrient-sources nutrient-uptake plantscultivarsgenetic-variation literature-reviews 10. Atrazine degradation in pesticide-contaminated soils: phytoremediation potential. Kruger, E. L., Anhalt, J. C., Sorenson, D., Nelson, B., Chouhy, A. L., Anderson, T. A., and Coats, J. R. Phytoremediation of soil and water contaminants. Washington, DC : American Chemical Society, 1997. p. 54-64. NAL Call #: QD1.A45-no. 664 Abstract: Studies were conducted in the laboratory to determine the fate of atrazine in pesticide-contaminated soils from agrochemical dealer sites. No significant differences in atrazine concentrations occurred in soils treated with atrazine i ndividually or combinations with metolachlor and trifluralin. In a screening study carried out in soils from four agrochemical dealer sites, rapid mineralization of atrazine occurred in three out of eight soils tested, with the greatest amount occurring i n Bravo rhizosphere soil (35% of the applied atrazine after 9 weeks). Suppression of atrazine mineralization in the Bravo rhizosphere soil did not occur with the addition of high concentrations of herbicide mixtures, but instead was increased. Plants had a positive impact on dissipation of aged Studies were conducted in the laboratory to determine the fate of atrazine in pesticide-contaminated soils from agrochemical dealer sites. No significant differences in atrazine concentrations occurred in soils treated with atrazine i ndividually or combinations with metolachlor and trifluralin. In a screening study carried out in soils from four agrochemical dealer sites, rapid mineralization of atrazine occurred in three out of eight soils tested, with the greatest amount occurring i n Bravo rhizosphere soil (35% of the applied atrazine after 9 weeks). Suppression of atrazine mineralization in the Bravo rhizosphere soil did not occur with the addition of high concentrations of herbicide mixtures, but instead was increased. Plants had a positive impact on dissipation of aged atrazine in soil, with significantly less atrazine extractable from Kochia-vegetated soils than from nonvegetated soils. 11. Bacterial inoculants of forage grasses that enhance degradation of 2-chlorobenzoic acid in soil. Siciliano, S. D. and Germida, J. J. Environ toxicol chem. 16: 6 pp. 1098-1104. (June 1997). NAL Call #: QH545.A1E58 Descriptors: polluted-soils bioremediationAbstract: Biological remediation of contaminated soil is an effective method of reducing risk to human and ecosystem health. Bacteria and plants might be used to enhance remediation of soil pollutants in situ. This study assessed the potential of bacteria (12 isolates), plants (16 forage grasses), and plant-bacteria associations (selected pairings) to remediate 2-chlorobenzoic acid (2CBA)-contaminated soil. Initially, grass viability was assessed in 2CBA-contaminated soil. Soil was contaminated wi th 2CBA, forage grasses were grown under growth chamber conditions for 42 or 60 d, and the 2CBA concentration in soil was determined by gas chromatography. Only five of 16 forage grasses grew in 2CBA-treated (816 mg/kg) soil. Growth of Bromus inermis had no effect on 2CBA concentration, whereas Agropyron intermedium, B. biebersteinii, A. riparum, and Elymus dauricus decreased 2CBA relative to nonplanted control soil by 32 to 42%. The 12 bacteria isolates were screened for their ability to promote the germ ination of the five grasses in 2CBA-contaminated soil. Inoculation of A. riparum with Pseudomonas aeruginos

Phytoremediation : 植物による環境/土壌浄化

Soil tillage practices can affect soil hydraulic properties and processes dynamically in space and time with consequent and coupled effects on chemical movement and plant growth. This literature review addresses the quantitative effects of soil tillage and associated management (e.g., crop residues) on the temporal and spatial variability of soil hydraulic properties. Our review includes incidental management effects, such as soil compaction, and natural sources of variability, such as topography. Despite limited research on space-time predictions, many studies have addressed management effects on soil hydraulic properties and processes relevant to improved understanding of the sources of variability and their interactions in space and time. Whether examined explicitly or implicitly, the literature includes studies of interactions between treatments, such as tillage and residue management. No-tillage (NT) treatments have been compared with various tillage practices under a range of conditions with mixed results. The trend, if any, is for NT to increase macropore connectivity while generating inconsistent responses in total porosity and soil bulk density compared with conventional tillage practices. This corresponds to a general increase in ponded or near-zero tension infiltration rates and saturated hydraulic conductivities. Similarly, controlled equipment traffic may have significant effects on soil compaction and related hydraulic properties on some soils, but on others, landscape and temporal variability overwhelm wheel-track effects. Spatial and temporal variability often overshadows specific management effects, and several authors have recognized this in their analyses and interpretations. Differences in temporal variability depend on spatial locations between rows, within fields at different landscape positions, and between sites with different climates and dominant soil types. Most tillage practices have pronounced effects on soil hydraulic properties immediately following tillage application, but these effects can diminish rapidly. Long-term effects on the order of a decade or more can appear less pronounced and are sometimes impossible to distinguish from natural and unaccounted management-induced variability. New standards for experimental classification are essential for isolating and subsequently generalizing space-time responses. Accordingly, enhanced methods of field measurement and data collection combined with explicit spatio-temporal modeling and parameter estimation should provide quantitative predictions of soil hydraulic behavior due to tillage and related agricultural management.

Tillage effects on soil hydraulic properties in space and time: State of the science

The global temperature has been increasing over the years due to climate change that, directly or indirectly, affects water and energy consumptions in the agriculture sector. The application of mulching practices reduces soil evaporation, conserves soil moisture, suppresses weed growth, controls soil structure and temperature, influences soil micro-organisms, and is aesthetically pleasing. This study has reviewed 189 published research papers, which described the effects of various mulching materials and methods on soil and environment that influence crop productivity. This paper describes the extent of influence of different mulching materials and methods on the hydrothermal environment of soils. It is imperative to know the processes that control soil environments under various mulching conditions and the effects of mulching materials on crop yield, productivity and water use efficiency. These issues of mulching are the prime concerns of this review study. Plastic mulching materials have a greater importance than the organic ones to control soil environment and increase crop yield. But, the organic mulching materials are inexpensive and environment friendly. The selection of an appropriate mulching material is, however, guided by crop type, crop management practices and climatic conditions. Future research is needed on the effects of low-cost biodegradable mulching materials on microclimate modifications, soil biota, soil fertility, crop growth and crop yields.

Recent advances in mulching materials and methods for modifying soil environment

Stable isotopes and plant carbon-water relations

The Earth’s history is punctuated by large explosive eruptions that eject large quantities of magma and silicate rock fragments into the atmosphere. These tephra particles can sometimes be dispersed across millions of square kilometres or even entire continents. The interaction of tephra with or in receiving environments may induce an array of physical, chemical and biological effects. The consequences for affected systems and any dependent communities may be chronic and localised in the event of frequent, small eruptions, while larger and rarer events may have acute, regional-scale impacts. It is, therefore, necessary to document the range of possible impacts that tephra may induce in receiving environments and any resulting effects in interconnected systems. We collate results from many studies to offer a detailed multidisciplinary and interdisciplinary review of the immediate post-eruptive effects of tephra emission into the atmosphere, onto vegetation, soil or ice/snow surfaces and in aquatic systems. We further consider the repercussions that may be induced in the weeks to years afterwards. In the atmosphere, tephra can influence cloud properties and air chemistry by acting as ice nuclei (IN) or by offering sites for heterogeneous reactions, respectively. Tephra on vegetation causes physical damage, and sustained coverage may elicit longer-term physiological responses. Tephra deposits on soils may alter their capacity to exchange gas, water and heat with the atmosphere or may have a specific chemical effect, such as nutrient input or acidification, on sensitive soils. Tephra deposition onto snow or ice may affect ablation rates. Rivers and lakes may experience turbidity increases and changes in their morphology as a result of fallout and prolonged (months or years) erosion from the tephra-covered catchment. In the first weeks after deposition, tephra leaching may affect river chemistry. The abundance and speciation of phytoplankton populations in lakes may be altered by tephra-induced changes in water chemistry or sediment–water nutrient cycling. In the oceans, tephra deposition may fertilise Fe-limited waters, with potential impacts on the global carbon cycle. Embracing the full complexity of environmental effects caused by tephra fall demands a renewed investigative effort drawing on interdisciplinary field and laboratory studies, combined with consideration of the interconnectivity of induced impacts within and between different receiving environments.

The immediate environmental effects of tephra emission

Influence of the thickness and grain size of tephra mulch on soil water evaporation

Assessing the suitability of saline wastewaters for irrigation of Citrus spp.: Emphasis on boron and specific-ion interactions

Volcanic materials as mulches for water conservation

Biochar and earthworms working in tandem: Research opportunities for soil bioremediation.

Natural soil salinization and sodication processes that restrict or prevent crop growth are commonplace in arid regions. In saline-sodic soils of arid parts of the Canarian archipelago, where annual rainfall is <150 mm, a traditional agriculture system has been developed using basaltic tephra mulch. This system has enabled dry crop farming to take place even under the most adverse of natural conditions. The present work compares the salinity-sodicity in the cultivated layer of soils that were covered with 10 to 15 cm of tephra mulch as recently as 20 yr ago with that of adjacent unmulched soils. These soils have never been irrigated. Results show significant differences in electrical conductivity (EC) and exchangeable Na percentage (ESP). The natural, unmulched soils are extremely saline-sodic (EC se 43 dSm -1 , ESP 44), whereas the mulched soils are neither saline nor sodic (EC se 1.5 dsm -1 , ESP 9). The reduction in salinity and sodicity in the mulched soils was related to the change in soil moisture regime caused by the mulch covering, which increases infiltration and reduces evaporation and upward movement of Na + and other salts. Probably, additional water from dew could also contribute. The greater dilution of soil solution through increased soil water content, increased Na + leaching and the dissolution of calcium salts may account for the desalinization and desodication. These results were confirmed through monitoring of a soil from the moment it was originally covered with tephra. They also revealed salt leaching process is a short-term. Tephra mulching can; therefore, be a highly efficient technique for the recovery of saline-sodic soils under dry farming conditions.

Francisco J. Díaz

Papers

Influence of the thickness and grain size of tephra mulch on soil water evaporation

Assessing the suitability of saline wastewaters for irrigation of Citrus spp.: Emphasis on boron and specific-ion interactions

Volcanic materials as mulches for water conservation

Biochar and earthworms working in tandem: Research opportunities for soil bioremediation.

Use of Volcanic Mulch to Rehabilitate Saline-Sodic Soils