F
Fred A. Faloona
Researcher at Cetus Corporation
Publications - 5
Citations - 19105
Fred A. Faloona is an academic researcher from Cetus Corporation. The author has contributed to research in topics: Polymerase & Hot start PCR. The author has an hindex of 5, co-authored 5 publications receiving 18626 citations.
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Journal ArticleDOI
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Randall Keichi Saiki,Stephen J. Scharf,Fred A. Faloona,Kary B. Mullis,Glenn Thomas Horn,Henry A. Erlich,Norman Arnheim +6 more
TL;DR: Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia, using primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase of target DNA copies.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
Kary B. Mullis,Fred A. Faloona,Stephen J. Scharf,Randall Keichi Saiki,Glenn Thomas Horn,Henry A. Erlich +5 more
TL;DR: An alternative method for the synthesis of specific DNA sequences is explored that involves the reciprocal interaction of two oligonucleotides and the DNA polymerase extension products whose synthesis they prime, when they are hybridized to different strands of a DNA template in a relative orientation such that their extension products overlap.
Book ChapterDOI
9 – Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: In this article, a specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction was discussed, where a source of DNA including the desired sequence was denatured in the presence of a large molar excess of two oligonucleotides and the four deoxyribonucleoside triphosphates.