F
Friedrich Fauser
Researcher at Carnegie Institution for Science
Publications - 25
Citations - 2301
Friedrich Fauser is an academic researcher from Carnegie Institution for Science. The author has contributed to research in topics: CRISPR & Genome engineering. The author has an hindex of 13, co-authored 23 publications receiving 1835 citations. Previous affiliations of Friedrich Fauser include BASF Plant Science & Karlsruhe Institute of Technology.
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Journal ArticleDOI
Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
TL;DR: It is shown that only the nuclease but not the nickase is an efficient tool for NHEJ-mediated mutagenesis in plants and the Cas9 nickase has the potential to become an important tool for genome engineering in plants.
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The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny
TL;DR: It is demonstrated that this Cas9 paired nickase strategy has a mutagenic potential comparable with that of the nuclease, while the resulting mutations are mostly deletions, and the stable inheritance of such mutations in A. thaliana is demonstrated.
Journal ArticleDOI
Highly efficient heritable plant genome engineering using Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus.
TL;DR: Codon-optimised Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus can both be used to induce error-prone non-homologous end-joining-mediated targeted mutagenesis in the model plant Arabidopsis thaliana at frequencies at least comparable to those that have previously been reported for the S. pyogenes CRISPR/Cas system.
Journal ArticleDOI
Synthetic nucleases for genome engineering in plants: prospects for a bright future.
Holger Puchta,Friedrich Fauser +1 more
TL;DR: The recently discovered RNA-based CRISPR/Cas system, a new tool to induce multiple DSBs, and sophisticated technical applications, such as the in planta gene targeting system, are further steps in this development.
Journal ArticleDOI
In planta gene targeting
Friedrich Fauser,Nadine Roth,Michael Pacher,Gabriele Ilg,Rocio Sanchez-Fernandez,Christian Biesgen,Holger Puchta +6 more
TL;DR: A highly efficient GT system that is suitable for all transformable plants regardless of transformation efficiency is described, achieved in Arabidopsis thaliana by expression of a site-specific endonuclease that not only cuts within the target but also the chromosomal transgenic donor, leading to an excised targeting vector.