Showing papers by "George G. Guilbault published in 1994"
TL;DR: Methods of antibody immobilization on crystals and several applications are reported including microgravimetric immunoassays, microbial toxins and other contaminants.
Abstract: The development of piezoelectric (PZ) devices as immunosensors is reviewed. The recent advances in sensor design have stimulated great interest in PZ technology and facilitated diverse applications in a variety of matrices. Methods of antibody immobilization on crystals and several applications are reported including microgravimetric immunoassays, microbial toxins and other contaminants.
106 citations
TL;DR: Fibre-optic biosensors were constructed and the H2O2 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase.
46 citations
TL;DR: A fiber-optic biosensor based on the immobilization of sulphite oxidase and peroxidase on different membranes, and subsequent detection of luminol chemiluminescence has been developed.
22 citations
TL;DR: Several analytical characteristics of this sulfite biosensor were investigated including dynamic range, reversibility, reproducibility, stability and selectivity, which were in good agreement with the standard AOAC method.
19 citations
TL;DR: An alcohol electrode which had been optimized to give an extended linear response to alcohol, eliminate the classical electrochemical interferences of hydrogen peroxide-based electrodes and be pH independent, was used to measure the alcohol concentration in beverage samples both in batch and FIA (wall jet) mode.
Abstract: An alcohol electrode which had been optimized1 to give an extended linear response to alcohol, eliminate the classical electrochemical interferences of hydrogen peroxide-based electrodes and be pH independent, was used to measure the alcohol concentration in beverage samples both in batch and FIA (wall jet) mode. The results obtained were compared to a spectrophotometric measurement used with a Sigma kit. It was found that the batch measurements were more accurate than the kit and FIA, although very good precision could be obtained with FIA. The method is rapid, easy to perform and requires no sample preparation.
18 citations
TL;DR: Enzyme electrodes for the amperometric measurement of urea were prepared by co-immobilizing l-glutamate dehydrogenase and urease onto an Immobilon-AV affinity membrane with attachment to a glassy carbon electrode.
12 citations
7 citations
TL;DR: The analytical characteristics of the microelectrodes, including calibration curves, apparent KM′, pH response curves, stability and selectivity over enzymatic interferences were determined and the response was linear in the concentration range.
Abstract: Glutathione micro-enzyme sensors were developed based on the immobilization of glutathione oxidase at the tip of a 25 μm Pt wire sealed in glass. An inner membrane constructed from cellulose acetate (CA) was deposited onto the tip of the Pt microelectrode prior to enzyme immobilization with glutaraldehyde. The final outer diameter of the microelectrodes is approximately 30 μm. The analytical characteristics of the microelectrodes, including calibration curves, apparent KM′, pH response curves, stability and selectivity over enzymatic interferences were determined. The response was linear in the concentration range 1.0 × 10−5 M - 1.9 × 10−4 M glutathione, reaching 95% steady-state current in 15–20 seconds. The microelectrodes were useful for more than two months.
1 citations