Fractionation of MOPC 41 DL-1 tumors revealed that the mRNA for the light chain of immunoglobulin is localized exclusively in membrane-bound ribosomes. It was shown that the translation product of isolated light chain mRNA in a heterologous protein-synthesizing system in vitro is larger than the authentic secreted light chain; this confirms similar results from several laboratories. The synthesis in vitro of a precursor protein of the light chain is not an artifact of translation in a heterologous system, because it was shown that detached polysomes, isolated from detergent-treated rough microsomes, not only contain nascent light chains which have already been proteolytically processed in vivo but also contain unprocessed nascent light chains. In vitro completion of these nascent light chains thus resulted in the synthesis of some chains having the same mol wt as the authentic secreted light chains, because of completion of in vivo proteolytically processed chains and of other chains which, due to the completion of unprocessed chains, have the same mol wt as the precursor of the light chain. In contrast, completion of the nascent light chains contained in rough microsomes resulted in the synthesis of only processed light chains. Taken together, these results indicate that the processing activity is present in isolated rough microsomes, that it is localized in the membrane moiety of rough microsomes, and, therefore, that it was most likely solubilized during detergent treatment used for the isolation of detached polysomes. Furthermore, these results established that processing in vivo takes place before completion of the nascent chain. The data also indicate that in vitro processing of nascent chains by rough microsomes is dependent on ribosome binding to the membrane. If the latter process is interfered with by aurintricarboxylic acid, rough microsomes also synthesize some unprocessed chains. The data presented in this paper have been interpreted in the light of a recently proposed hypothesis. This hypothesis, referred to as the signal hypothesis, is described in greater detail in the Discussion section.

/pdf/transfer-of-proteins-across-membranes-i-presence-of-1t5awzhhje.pdf

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Polyamines in rapid growth and cancer

I. Introduction II. The Regulation of Thyroid Function in Normal Pregnancy A. The thyroid hormone transport proteins B. The thyroid hormones 1. Total thyroid hormones 2. Free thyroid hormones 3. Peripheral metabolism of thyroid hormones C. The serum levels of thyroglobulin (TG) D. The metabolism of iodine E. The hypothalamic-pituitary control of thyroid function and the role of hCG 1. Hypothalamic-pituitary-thyroid axis (HPTA) 2. Regulation of serum TSH 3. Thyrotropic action of hCG F. A global view of thyroidal economy in pregnancy III. Pathological Alterations of Thyroidal Regulation Associated with Pregnancy A. IDD 1. Consequences of iodine deficiency during pregnancy 2. Assessment of increased thyroidal stimulation 3. Gestational goitrogenesis and its prevention by iodine supplementation 4. Consequences of iodine deficiency for the offspring B. Hypothyroidism and pregnancy 1. Fertility and pregnancy outcome in hypothyroid women 2. Thyroid hormone replacement in the hypothyroid pregnant woman 3. Subclin...

/pdf/the-regulation-of-thyroid-function-in-pregnancy-pathways-of-1jacooaoul.pdf

The Regulation of Thyroid Function in Pregnancy: Pathways of Endocrine Adaptation from Physiology to Pathology

accademiche [38]. Ada [45]. Adrian [45]. African [56]. Age [39, 49, 61]. Al [23]. Al-Rawi [23]. Aldous [68]. Alex [15]. Allure [46]. America [60, 66]. American [49, 69, 61, 52]. ancienne [25]. Andreas [28]. Angela [42]. Animals [16]. Ann [26]. Anna [19, 47]. Annotated [46]. Annotations [28]. Anti [37]. Anti-Copernican [37]. Antibiotic [64]. Anxiety [51]. Apocalyptic [61]. Archaeology [26]. Ark [36]. Artisan [32]. Asylum [48]. Atri [54]. Audra [65]. Australia [41]. Authorship [15]. Axelle [29].

/pdf/a-complete-bibliography-of-publications-in-isis-2000-2009-1nklqbzyfs.pdf

A Complete Bibliography of Publications in Isis, 2000{2009

Intracellular phospholipases A.

Chapter 1: Obstetric Management of the High-Risk Patient Chapter 2: Diabetes in Pregnancy Chapter 3: Hypertensive Disorders in Pregnancy Chapter 4: Hematologic Aspects of Pregnancy Chapter 5: Venous Thromboembolism During Pregnancy Chapter 6: Pregnancy and Cardiovascular Disease Chapter 7: Thyroid Diseases Chapter 8: Pituitary and Adrenal Disorders of Pregnancy Chapter 9: Calcium Homeostasis and Disorders of Calcium Metabolism During Pregnancy and Lactation Chapter 10: Clinical Genetics Chapter 11: Ethical Issues in Obstetrics Chapter 12: Emergency Management of the Obstetric Patient Chapter 13: Renal Diseases Chapter 14: Gastrointestinal Complications Chapter 15: Liver Diseases Chapter 16: Bacterial, Fungal, and Parasitic Disease Chapter 17: Viral Infections Chapter 18: Pulmonary Diseases Chapter 19: Neurologic Complications Chapter 20: Rheumatic Disease in Pregnancy Chapter 21: Immunology of Pregnancy Chapter 22: The Skin in Pregnancy Chapter 23: Neoplastic Diseases Chapter 24 : Psychiatric Complications Chapter 25: Substance Abuse

Medical Complications During Pregnancy

Ten normal males were given 100 mug TRH, and blood samples obtained for serum TSH and serum prolactin. After a period of at least one week, the TRH test was repeated while the patients were receiving a dopamine infusion. Both the TSH and prolactin response to TRH were inhibited by dopamine. Dopaminergic neurons may act through the pituitary-portal system to play a role in the regulation of TSH and prolactin secretion.

Dopamine infusion acutely inhibits the tsh and prolactin response to trh

Dopamine infusion consistently elevated baseline serum growth hormone values in normal males in contrast to a suppression of baseline serum prolactin and perhaps TSH values. The mean peak serum TSH concentration in response to 100 fjig TRH was 14 ± 2 μU/ml in normal males and fell to 7 ± 2 μU/ml when a dopamine infusion was begun 5 min before the TRH bolus and continued for 120 minutes. Under similar conditions, the mean peak serum prolactin response (36 ± 3 ng/ml) was obliterated and was markedly attenuated when the dopamine infusion was begun 5 min after the TRH bolus (17 ± 2 ng/ml) (P < .01). Administration of 100 μg of T3 4 h previously resulted in a mean peak serum prolactin concentration of 46 ± 6 ng/ml in response to TRH ad ministered 5 min before a 60 min dopamine infusion, compared to 17 ± 2 ng/ml without triiodothyronine (P < .01). Serum growth hormone rose to a mean peak value of 13 ± 2 ng/ml at 60 min in response to dopamine from a mean baseline value of 1 ±0.1 ng/ml (P < .001). When 100 μg of...

TRH and Dopamine Interactions Affecting Pituitary Hormone Secretion

Over two billion people worldwide are at risk for the spectrum of disorders known as 'The Iodine Deficiency Disorders'. 1-10 per cent will suffer cretinism; 5-30 per cent will have some sort of brain damage or neurological impairment and 30-70 per cent will be hypothyroid. The causes of iodine deficiencies can be considered from both simplistic and more complex perspectives: from the leaching of iodine from soil resulting in crops with low iodine content to malnutrition resulting in impaired iodine absorption. Poor dietary diversification and impoverished socio-economic development can also lead to iodine deficiencies. Although it is possible to diagnose and treat deficiencies, there is still an ongoing dialogue regarding the detailed molecular pathology of iodine homeostatis, how hypothyroidism impacts the body tissues, and efficient diagnosis and treatment of the Iodine Deficiency Disorders. This Handbook provides a resource of information on the various pathways and processes based on different countries or diseases. Because there is a constant flow of new information on iodine and related disorders, the goal of this Handbook is to provide a base of scientific information upon which additional knowledge can be applied. This title provides important information on one of the most common micro-nutrient deficiencies in the world, the most important 'single nutrient-multiple consequences' paradigm today. It includes information on iodine-related diseases, including those that are common, preventable and treatable. It provides insight from a broad perspective of viewpoints - from subcellular transports to economic impact.

Comprehensive handbook of iodine: nutritional, biochemical, pathological and therapeutic aspects.

PURPOSEDetection of mRNA transcripts for thyroglobulin (TG), thyroid peroxidase (TPO) and RET/PTC1 in the peripheral blood of patients with thyroid disease.PATIENTS AND METHODSTG, TPO, and RET/PTC1 mRNA were analyzed in 52 peripheral-blood samples from 44 patients diagnosed with thyroid carcinoma (24 patients), adenoma (five patients), and nodular hyperplasia (15 patients) by reverse transcription-polymerase chain reaction (RT-PCR).RESULTSTG and TPO were identified in 13 patients (54.2%) with thyroid carcinoma, which includes five of eight patients with no clinical evidence of disease at the time of blood collection. Four of 5 patients had cervical lymph node metastases and/or extrathyroid extension at the time of the initial surgery. RET/PTC1 mRNA was detected in the peripheral blood of only one patient with papillary thyroid carcinoma. This sample was also positive for TG and TPO. TG and TPO were detected in two patients (10%) with benign thyroid nodules. All positive samples from patients with benign t...

Gerard N. Burrow

Papers

Medical Complications During Pregnancy

Dopamine infusion acutely inhibits the tsh and prolactin response to trh

TRH and Dopamine Interactions Affecting Pituitary Hormone Secretion

Comprehensive handbook of iodine: nutritional, biochemical, pathological and therapeutic aspects.

Detection of thyroglobulin, thyroid peroxidase, and RET/PTC1 mRNA transcripts in the peripheral blood of patients with thyroid disease.