Gi-Sun Im

TL;DR: The production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out is reported, paving the way for xenotransplantation of pigs from clonal fetal fibroblast cell lines.

TL;DR: An unexpectedly high rate of spontaneous loss of GGTA1 function was observed, with the vast majority of null cells resulting from loss of the WT allele, and congenital abnormalities observed in the heterozygous NT animals did not reappear in the serially produced null animals.

TL;DR: Nisi from a genetically modified fibroblast cell line to porcine oocytes are transferred, matured in vitro under defined culture conditions, to create piglets expressing enhanced green fluorescent protein, which represents a next step towards creating swine with more useful genetic modifications.

TL;DR: The data suggest that the combination of transduction of somatic cells by a replication defective vector with the nuclear transfer of colchicine‐treated donors is an alternative to produce transgenic pigs and the tissues expressing EGFP from descendents of this pig may be very useful in future studies using pigs that require genetically marked cells.

TL;DR: The development of porcine nuclear transfer embryos reconstructed by using fetal fibroblasts as compared to embryos produced by in vitro fertilization was investigated to identify specific apoptotic pathways and intervene in these pathways to improve development.