The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.

Integrated Apparatus for Performing Nucleic Acid Extraction and Diagnostic Testing on Multiple Biological Samples

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

Rare cell analysis using sample splitting and dna tags

Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.

Lateral flow assays

This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Integrated system for processing microfluidic samples, and method of using same

The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.

Devices and methods for enrichment and alteration of circulating tumor cells and other particles

Chronic kidney disease (CKD) and acute kidney injury (AKI) are interconnected and the presence of one is a risk for the other. CKD is an important predictor of AKI after exposure to nephrotoxic drugs or major surgery, whereas persistent or repetitive injury could result in the progression of CKD. This brings new perspectives to the diagnosis and monitoring of kidney diseases highlighting the need for a panel of kidney-specific biomarkers that reflect functional as well as structural damage and recovery, predict potential risk and provide prognosis. This article discusses the kidney-specific biomarkers, symmetric dimethylarginine (SDMA), clusterin, cystatin B, and inosine.

Kidney Disease and the Nexus of Chronic Kidney Disease and Acute Kidney Injury: The Role of Novel Biomarkers as Early and Accurate Diagnostics.

A complex including magnetic particle bound to a metal colloid. The complex may be part of a reagent for use in a method for determining analytes. The reagent may include a binding partner specific for an analyte. The reagent may further include a first label that is distinguishable from a second label that is used to detect the analyte. The reagent is used in kits and methods for detecting analytes in samples. The methods include immunoassay methods, including method where the first label is used to calibrate the assay.

Lateral flow assay and device using magnetic particles

A method and a kit for determining the presence or amount of analytes in samples over a broad potential concentration range or samples The method and kit incorporate chemiluminescent and fluorescent labels conjugated to a specific binding partner for the analyte for sandwich assays, or to the analyte or an analog of the analyte for competitive assays The conjugates are mixed with the sample and the labels are detected simultaneously or sequentially

Dual standard curve immunoassay

ObjectivesMeloxicam therapy may benefit cats with degenerative joint disease, and retrospective studies suggest it could slow kidney disease progression and increase survival This study aimed to p

Effects of low-dose meloxicam in cats with chronic kidney disease.

An apparatus, device, and method for laterally moving or assisting in the lateral movement of at least one of a liquid sample and a reagent along a porous carrier matrix is disclosed. The method comprises contacting the matrix with the at least one of the sample and the reagent and exposing the at least one of a liquid sample and a reagent to a centrifugal force, thereby moving or assisting in the lateral movement of the at least one of a liquid sample and a reagent along the porous carrier matrix.

Giosi Farace

Papers

Kidney Disease and the Nexus of Chronic Kidney Disease and Acute Kidney Injury: The Role of Novel Biomarkers as Early and Accurate Diagnostics.

Lateral flow assay and device using magnetic particles

Dual standard curve immunoassay

Effects of low-dose meloxicam in cats with chronic kidney disease.

Lateral flow assay using centrifugal force