Showing papers by "Gordon L. Warren published in 2005"
TL;DR: It is delineated that signaling through CCR2 is involved in muscle precursor cell activities necessary for complete and rapid regeneration of injured skeletal muscle.
Abstract: SPECIFIC AIMSWe hypothesized that signaling through CCR2, a major receptor for monocyte chemoattractant protein-1 MCP-1 (CCL2), affects degeneration/regeneration after skeletal muscle injury via indirect and direct effects on muscle precursor cells. We characterized localization of CCR2 in regenerating muscle [freeze-injured mouse tibialis anterior (TA) muscle] and compared cellular/molecular characteristics of skeletal muscle regeneration in CCR2-deficient vs. wild-type (WT) mice.PRINCIPAL FINDINGS1. CCR2 cellular localization in injured skeletal muscleTo determine whether expression of CCR2 is localized to macrophages and/or muscle precursor cells in injured muscle, we conducted double immunostaining for CCR2 and Mac-3, a marker of activated monocytes/macrophages, or myogenin, a marker of activated and differentiating muscle precursor cells. CCR2 was found to be localized to the membrane of cells expressing cytoplasmic localized Mac-3 (Fig. 1⤻ A) or nuclear localized myogenin (Fig. 1B⤻ ), demonstrating ...
167 citations
TL;DR: Additional calories from CHO or P above that provided in sports drinks does not improve subsequent performance after recovery; but less soreness suggests benefits with CHO-P.
Abstract: Post-exercise nutrition is critical to facilitate recovery from training. To determine if added protein (P) or increased carbohydrate (CHO) differentially improves recovery, eight runners ingested: 6% CHO (CHO6), 8% CHO + 2% protein (CHO-P), and isocaloric 10% CHO (CHO10) following a 21-km run plus treadmill run to fatigue (RTF) at 90% VO2max. RTF was repeated after 2 h recovery. After 24 h, a 5 km time trial was performed. Insulin and blood glucose were higher (P < 0.05) following CHO10 compared to CHO-P and CHO6, but did not affect improvement from the first to second RTF (29.6% +/- 6, 40.5% +/- 8.8, 40.5% +/- 14.5) or 5 km time (1100 +/- 36.3, 1110 +/- 37.3, 1118 +/- 36.5 s). CK was not different, but perceived soreness with CHO-P (2.1 +/- 0.5) was lower than CHO10 (5.2 +/- 0.7). Additional calories from CHO or P above that provided in sports drinks does not improve subsequent performance after recovery; but less soreness suggests benefits with CHO-P.
93 citations
TL;DR: This study provides essential, comprehensive baseline data for future studies on age-related muscle dysfunction in the female mouse and indicates that a deterioration in soleus muscle quality of female mice occurred beginning around the age of ovarian failure.
78 citations
7 citations