Guillaume Hummel

TL;DR: It is demonstrated that the Arabidopsis RNases T2, called RNS, are key players of both long and short tRFs biogenesis, and an evolutionary conserved feature of these enzymes in eukaryotes is suggested.

TL;DR: Two modifications to standard RNA-seq methods are combined by treating with the demethylating enzyme AlkB and ligating with tRNA-specific adapters in order to sequence tRNAs from four species of flowering plants, a group that has been shown to have some of the most extensive rates of post-transcriptional tRNA modifications.

TL;DR: The different actors implicated in the first step of transfer RNA biogenesis in eukaryotes are summarized: cis‐elements, trans‐factors, genomic contexts, epigenetic environments and finally three‐dimensional organization of nuclear genomes.

TL;DR: This work demonstrates that both genomic and epigenomic contexts are key players in the regulation of tDNAs transcription and conservation of most of these regulatory processes suggests that this pioneering work in Arabidopsis can provide new insights into theregulation of RNA Pol III transcription in other organisms, including vertebrates.

TL;DR: The latest development in the understanding of plant spatial genome structures and different levels of organization and their functional implications are reviewed and unaddressed research axes are proposed to investigate functional links between chromatin-like dynamics and transcriptional regulation within organellar nucleoids.