Stéphanie Lalande

TL;DR: It is demonstrated that the Arabidopsis RNases T2, called RNS, are key players of both long and short tRFs biogenesis, and an evolutionary conserved feature of these enzymes in eukaryotes is suggested.

TL;DR: It is demonstrated that the organellar tRFs are not found within chloroplasts or mitochondria but rather accumulate outside the organelles, suggesting that some organellartRFs could play regulatory functions within the plant cell and may be part of a signaling pathway.

TL;DR: The present knowledge on the biogenesis and function of tRFs in various organisms is reviewed, suggesting that these cleavage products of tRNAs now potentially emerge as a new class of small non-coding RNAs (sncRNAs) and are suspected to have important regulatory functions.

TL;DR: It is shown that populations of long (30–35 nT) or short (19–25 nt) tRFs produced from Arabidopsis tRNAs can inhibit in vitro translation of a reporter gene.

TL;DR: An optimized protocol to deliver small noncoding RNAs into Arabidopsis thaliana protoplasts is described and an example of application is provided by demonstrating the incorporation of a 20 nt long smallNoncoding RNA deriving from the 5' extremity of an A. thalianA cytosolic alanine tRNA into freshly isolated protoplast.