Showing papers by "Gyun Min Lee published in 1990"
TL;DR: Cells in serum‐free media showed a decrease in antibody productivity, and it completely disappeared in IMDM‐based low protein medium, but this loss of antibody productivity was not observed when the cells were immobilized in alginate beads, and in fact, immobilization enhanced the specific MAb productivity.
Abstract: Hybridoma cells (S3H5/gamma2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease in antibody productivity, and it completely disappeared in IMDM-based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.
87 citations
TL;DR: Phosphate concentration in RPMI 1640 medium is sufficiently high to dissolve the alginate gel and can be used to eliminate the step of citrate buffer treatment required for reliquefying the interior alginated gel.
Abstract: The method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPMI 1640 medium. Phosphate concentration in RPMI 1640 medium is sufficiently high to dissolve the alginate gel and, thereby, can be used to eliminate the step of citrate buffer treatment required for reliquefying the interior alginate gel.
13 citations