Detection of Glycosides and Other Carbohydrate Compounds on Paper Chromatograms

Buffered Filter Paper Chromatography of Amino Acids

Paper-based chemical and biological sensors: Engineering aspects.

A technique with subpicomolar sensitivity was developed for analyzing O-linked oligosaccharides released from glycoproteins separated by gel electrophoresis. The protocol involves gel electrophoresis, electroblotting to poly(vinylidene fluoride) membrane, reductive β-elimination, and analysis of released oligosaccharides by liquid chromatography coupled to negative ion electrospray mass spectrometry. It was also found that N-linked oligosaccharides could be recovered under the same conditions, found both as free oligosaccharides and as distinct glycopeptides created from reductive cleavage of the protein backbone, giving some information on site-specific glycosylation. The method was used to demonstrate that the difference between human α-2HS-glycoprotein isoforms separated by 2D-gel electrophoresis was partially due to sialylation of both O-linked and N-linked oligosaccharides. It was also shown that both acidic and neutral oligosaccharides could be recovered and analyzed simultaneously from high molecul...

Small-Scale Analysis of O-Linked Oligosaccharides from Glycoproteins and Mucins Separated by Gel Electrophoresis

Quantitative Determination of Amino Acids on Filter Paper Chromatograms by Direct Photometry

Consden, Gordon and Martin reported a very ingenious method for the separation of amino acids by filter paper chromatography in which phenol or some other appropriate organic solvent sweeps the applied amino acids along the filter paper, capillarity causing the organic solvent to move. The percentage of light intermission along the chromatogram is measured and this transmission has been plotted on semi-log graph paper against the distance along the filter paper strips, and the areas of the segments above the plotted curve measured with a planimeter. It has been found that the areas so determined are over a limited concentration range a simple function of the concentration of the amino acids. The various factors which influence the reliability of this method are reported.

Hahn Jw

Papers

Filter paper chromatography.