The worldwide increases in both environmental damage and human population pressure have the unfortunate consequence that global food production may soon become insufficient to feed all of the world's people. It is therefore essential that agricultural productivity be significantly increased within the next few decades. To this end, agricultural practice is moving toward a more sustainable and environmentally friendly approach. This includes both the increasing use of transgenic plants and plant growth-promoting bacteria as a part of mainstream agricultural practice. Here, a number of the mechanisms utilized by plant growth-promoting bacteria are discussed and considered. It is envisioned that in the not too distant future, plant growth-promoting bacteria (PGPB) will begin to replace the use of chemicals in agriculture, horticulture, silviculture, and environmental cleanup strategies. While there may not be one simple strategy that can effectively promote the growth of all plants under all conditions, some of the strategies that are discussed already show great promise.

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Plant Growth-Promoting Bacteria: Mechanisms and Applications

Free-living plant growth-promoting rhizobacteria (PGPR) can be used in a variety of ways when plant growth enhancements are required. The most intensively researched use of PGPR has been in agriculture and horticulture. Several PGPR formulations are currently available as commercial products for agricultural production. Recently developing areas of PGPR usage include forest regeneration and phytoremediation of contaminated soils. As the mechanisms of plant growth promotion by these bacteria are unravelled, the possibility of more efficient plant-bacteria pairings for novel and practical uses will follow. The progress to date in using PGPR in a variety of applications with different plants is summarized and discussed here.

Applications of free living plant growth-promoting rhizobacteria

Endophytic bacteria reside within plant hosts without causing disease symptoms. In this study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop species and 27 prairie plant species. We determined several phenotypic properties and found approximately equal numbers of gram-negative and gram-positive isolates. In a greenhouse study, 28 of 86 prairie plant endophytes were found to colonize their original hosts at 42 days postinoculation at levels of 3.5 to 7.7 log(10) CFU/g (fresh weight). More comprehensive colonization studies were conducted with 373 corn and sorghum endophytes. In growth room studies, none of the isolates displayed pathogenicity, and 69 of the strains were recovered from corn or sorghum seedlings at levels of 8.3 log(10) CFU/plant or higher. Host range greenhouse studies demonstrated that 26 of 29 endophytes were recoverable from at least one host other than corn and sorghum at levels of up to 5.8 log(10) CFU/g (fresh weight). Long-range dent corn greenhouse studies and field trials with 17 wild-type strains and 14 antibiotic-resistant mutants demonstrated bacterial persistence at significant average colonization levels ranging between 3.4 and 6.1 log(10) CFU/g (fresh weight) up to 78 days postinoculation. Three prairie and three agronomic endophytes exhibiting the most promising levels of colonization and an ability to persist were identified as Cellulomonas, Clavibacter, Curtobacterium, and Microbacterium isolates by 16S rRNA gene sequence, fatty acid, and carbon source utilization analyses. This study defines for the first time the endophytic nature of Microbacterium testaceum. These microorganisms may be useful for biocontrol and other applications.

/pdf/isolation-and-characterization-of-endophytic-colonizing-xigcx1jtvw.pdf

Isolation and Characterization of Endophytic Colonizing Bacteria from Agronomic Crops and Prairie Plants

Summary
Growth and activity at low temperatures and possible physiological and ecological mechanisms underlying survival of fungi isolated from the cold Arctic and Antarctic are reviewed here. Physiological mechanisms conferring cold tolerance in fungi are complex; they include increases in intracellular trehalose and polyol concentrations and unsaturated membrane lipids as well as secretion of antifreeze proteins and enzymes active at low temperatures. A combination of these mechanisms is necessary for the psychrotroph or psychrophile to function. Ecological mechanisms for survival might include cold avoidance; fungal spores may germinate annually in spring and summer, so avoiding the coldest months. Whether spores survive over winter or are dispersed from elsewhere is unknown. There are also few data on persistence of basidiomycete vs microfungal mycelia and on the relationship between low temperatures and the predominance of sterile mycelia in tundra soils. Acclimation of mycelia is a physiological adaptation to subzero temperatures; however, the extent to which this occurs in the natural environment is unclear. Melanin in dark septate hyphae, which predominate in polar soils, could protect hyphae from extreme temperatures and play a significant role in their persistence from year to year.

https://nph.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1469-8137.2001.00177.x

Cold adaptation in Arctic and Antarctic fungi

Historically, agricultural production has relied on practices designed to manage nutrients, water, weeds, and crop diseases. Precision agriculture and integrated pest management programs have gone one step further by recognizing the need to target inputs where they are required in the field. The major objective of these programs has been to minimize adverse environmental impacts of intensive agriculture practices and reduce per unit production costs. This review surveys the literature, examining the manipulation of microbial (primarily bacterial) populations as linked to agricultural production, and discusses new approaches that involve the precision management of microorganisms in the agro-ecosystem. It is proposed that our understanding of plant–soil interactions can be greatly refined through the development of “smart” field technology, where real-time, computer-controlled electronic diagnostic devices can be used to monitor rhizosphere and plant health. We submit that “smart field” generated informati...

Managing Soil Microorganisms to Improve Productivity of Agro-Ecosystems

An antifreeze protein secreted to the growth medium by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 was purified to apparent homogeneity. The purified protein has a molecular...

Isolation and characterization of an antifreeze protein with ice nucleation activity from the plant growth promoting rhizobacterium Pseudomonas putida GR12-2

Hao Xu

Papers

Isolation and characterization of an antifreeze protein with ice nucleation activity from the plant growth promoting rhizobacterium Pseudomonas putida GR12-2