scispace - formally typeset
H

Happy K. Shieh

Researcher at National Chung Hsing University

Publications -  37
Citations -  1315

Happy K. Shieh is an academic researcher from National Chung Hsing University. The author has contributed to research in topics: Virus & Pasteurella multocida. The author has an hindex of 21, co-authored 37 publications receiving 1246 citations.

Papers
More filters
Journal ArticleDOI

Identification and subtyping of avian influenza viruses by reverse transcription-PCR.

TL;DR: The RT-PCR procedure developed is rapid and sensitive, and could be used for the identification and HA-subtyping of avian influenza virus in organ homogenates.
Journal ArticleDOI

Newcastle disease virus isolated from recent outbreaks in Taiwan phylogenetically related to viruses (genotype VII) from recent outbreaks in western Europe.

TL;DR: It is suspected that these outbreaks of Newcastle disease in Taiwan have constituted the fourth panzootic of ND, which is distinct from the third panZootic caused by the "pigeon PMV-1 viruses."
Journal ArticleDOI

Rapid differentiation of vaccine strains and field isolates of infectious laryngotracheitis virus by restriction fragment length polymorphism of PCR products.

TL;DR: The results suggested that both vaccine-like and vaccine-unlike ILT viruses were involved in the field outbreak of this disease, and that the PCR/RFLP procedure could serve as a fast and sensitive method for the detection and differentiation of vaccine strains and field isolates of ILT virus.
Journal ArticleDOI

Experimental infections of rabbits with rabbit haemorrhagic disease virus monitored by polymerase chain reaction.

TL;DR: Adult and 4-5-week-old rabbits were inoculated subcutaneously with rabbit haemorrhagic disease virus (RHDV) and antibody titres increased rapidly from one week PI onwards, peaked at approximately three weeks of age, and were maintained throughout the length of the experiment.
Journal ArticleDOI

Cloning of the gene and characterization of the enzymatic properties of the monomeric alkaline phosphatase (PhoX) from Pasteurella multocida strain X-73.

TL;DR: Characterization of the recombinant PhoX of Pasteurella multocida X-73 showed that it is a monomeric enzyme, activated by Ca(2+) and possibly secreted by the Tat pathway, and homologous sequences between Pho X and the phosphotriesterase and on the potential calcium-binding site of PhoZ are identified.