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JournalISSN: 0378-1097

Fems Microbiology Letters 

About: Fems Microbiology Letters is an academic journal. The journal publishes majorly in the area(s): Escherichia coli & Plasmid. It has an ISSN identifier of 0378-1097. Over the lifetime, 18996 publication(s) have been published receiving 608306 citation(s).
Topics: Escherichia coli, Plasmid, Gene, Mutant, Virulence


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Journal ArticleDOI
TL;DR: The system described here gives a direct and precise method for determining DNA base composition by reversed-phase high-performance liquid chromatography (HPLC).
Abstract: DNA base composition was determined by reversed-phase high-performance liquid chromatography (HPLC). DNA was hydrolysed into nucleosides with nuclease P1 and bacterial alkaline phosphatase. The mixture of nucleosides was applied to HPLC without any further purification. One determination by chromatography needed 2 μg of hydrolysed nucleosides and took only 8 min. The relative standard error of nucleoside analysis was less than 1%. The system described here gives a direct and precise method for determining DNA base composition.

2,459 citations

Journal ArticleDOI
TL;DR: 'BLAST 2 Sequences', a new BLAST-based tool for aligning two protein or nucleotide sequences, is described, utilizing the BLAST algorithm for pairwise DNA-DNA or protein-protein sequence comparison.
Abstract: 'BLAST 2 Sequences', a new BLAST-based tool for aligning two protein or nucleotide sequences, is described. While the standard BLAST program is widely used to search for homologous sequences in nucleotide and protein databases, one often needs to compare only two sequences that are already known to be homologous, coming from related species or, e.g. different isolates of the same virus. In such cases searching the entire database would be unnecessarily time-consuming. 'BLAST 2 Sequences' utilizes the BLAST algorithm for pairwise DNA-DNA or protein-protein sequence comparison. A World Wide Web version of the program can be used interactively at the NCBI WWW site (http://www.ncbi.nlm.nih.gov/gorf/bl2.++ +html). The resulting alignments are presented in both graphical and text form. The variants of the program for PC (Windows), Mac and several UNIX-based platforms can be downloaded from the NCBI FTP site (ftp://ncbi.nlm.nih.gov).

2,218 citations

Journal ArticleDOI
TL;DR: The results indicated that the criterion for isolation of phosphate solubilizers based on the formation of visible halo/zone on agar plates is not a reliable technique, and soil microbes should be screened in NBRIP broth assay for the identification of the most efficient phosphate soluble inorganic phosphates in liquid medium.
Abstract: A novel defined microbiological growth medium, National Botanical Research Institute's phosphate growth medium (NBRIP), which is more efficient than Pikovskaya medium (PVK), was developed for screening phosphate solubilizing microorganisms. In plate assay the efficiency of NBRIP was comparable to PVK; however, in broth assay NBRIP consistently demonstrated about 3-fold higher efficiency compared to PVK. The results indicated that the criterion for isolation of phosphate solubilizers based on the formation of visible halo/zone on agar plates is not a reliable technique, as many isolates which did not show any clear zone on agar plates solubilized insoluble inorganic phosphates in liquid medium. It may be concluded that soil microbes should be screened in NBRIP broth assay for the identification of the most efficient phosphate solubilizers.

1,510 citations

Journal ArticleDOI
TL;DR: Investigation of the recently described aerobic, extremely halophilic archaeobaterium, Halobacterium lacusprofundi showed that the major respiratory lipoquinones present were MK-8 and MK- 8(VIII-H2), a feature found in other members of the family Halobacteriaiaceae.
Abstract: The recently described aerobic, extremely halophilic archaeobaterium, Halobacterium lacusprofundi was subjected to lipid analysis so that comparisons could be made between existing lipid data and that of the new isolate. This investigation showed that the major respiratory lipoquinones present were MK-8 and MK-8(VIII-H2), a feature found in other members of the family Halobacteriaceae. The polar lipids comprised the diether derivatives of phosphatidly glycerol, phosphatidyl glycerophosphate, phosphatidly glycerosulphate, a diglycosyl diether and its sulphate derivative. The data presented shows that Halobacterium locusprofundi is related to Hb. saccharovorum and Hb. sodomense, and is in agreement with phylogenetic data.

1,370 citations

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Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
2021171
2020181
2019277
2018308
2017256
2016313