High throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexibility and open accessibility. This paper reports on the integration of a polydimethylsiloxane (PDMS) micronozzle array and bidirectional electrokinetic pumps driven by DC-biased AC voltages. Pt/Ti and indium tin oxide (ITO) electrodes were used to study the effect of DC bias and peak-to-peak voltage and electrodes in a low conductivity isotonic solution. The flow was bidirectionally controlled by changing the DC bias. A pump integrated with a micronozzle array was used to transport single HeLa cells into nozzle holes. The application of DC-biased AC voltage (100 kHz, 10 Vpp, and VDC: -4 V) provided a sufficient electroosmotic flow outside the nozzle array. This integration method of nozzle and pumps is anticipated to be a standard integration method. The operating conditions of DC-biased AC electrokinetic pumps in a biological buffer was clarified and found useful for cell manipulation.

https://www.mdpi.com/2072-666X/11/4/442/pdf

Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps.

Light-Induced Cellular Delivery and Analysis

Characterization of Cellular Optoporation Using Nanosecond Laser and Metal Pattern for Massively Parallel Intranuclear Delivery

With the increasing need of regenerative medicines, cell therapy, and biopharmaceutical drug, it is necessary to develop a method for massively parallel delivery and uniform transfection. This research explores the possibility of using $\mu$ CP (using cell adhesive and cell blocking agents) for printing massive number of cells on a substrate and using optoporation methods (nano-second pulsed radiation) to perforate cell membranes. With combining these two methods, a stand-alone micro-device capable of performing localized intracellular delivery will be developed. In this paper, we confirmed the generation of shock wave upon optoporation, tried intracellular delivery using Calcein, develop the stamp for microcontact printing and performed the printing.

Uniform Transfection: Shock Wave Generation in Laser Ablation and Microcontact Printing

https://link-springer-com-443.webvpn.jmu.edu.cn/content/pdf/10.1007%2F978-981-10-4857-9_2-1.pdf

Single-Cell Manipulation

The objective of this study is to develop a parallel intranuclear delivery method to obtain stable and homogeneous cells at 104 level. A laser irradiation optical system and a simple device were fabricated for evaluation of basic characteristics. Nanosecond pulse laser was used to irradiate cells cultured on a titanium thin film using the laser irradiation optical system. The performance of the optical system was evaluated by cell viability assay. We examined the possibility of fluorescent molecules delivery to single cells.

Harsh Gupta

Papers

Characterization of Cellular Optoporation Using Nanosecond Laser and Metal Pattern for Massively Parallel Intranuclear Delivery

Uniform Transfection: Shock Wave Generation in Laser Ablation and Microcontact Printing

Single-Cell Manipulation

Nanosecond Laser Irradiation on Cells Using Titanium Thin Film for Massively Parallel Cell Intranuclear Delivery