H
Haruhiko Kawasaki
Researcher at Osaka Prefecture University
Publications - 56
Citations - 1326
Haruhiko Kawasaki is an academic researcher from Osaka Prefecture University. The author has contributed to research in topics: Haloacetate dehalogenase & Gene. The author has an hindex of 21, co-authored 56 publications receiving 1279 citations.
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Journal ArticleDOI
Lack of homology between two haloacetate dehalogenase genes encoded on a plasmid from Moraxella sp. strain B.
TL;DR: The dehalogenases H-1 and H-2 have no enzyme-evolutionary relationship, and the deduced amino acid sequence of the dehH1 gene showed significant similarity to those of three hydrolases of Pseudomonas putida and a haloalkane dehalagenase of Xanthobacter autotrophicus.
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Esterification of ferulic acid with polyols using a ferulic acid esterase from Aspergillus niger.
TL;DR: The enzyme responsible for the esterification was purified and characterized, and was found to be quite similar to an A. niger ferulic acid esterase (FAE-III) in terms of molecular mass, pH and temperature optima, substrate specificity on synthetic substrates, and the N-terminal amino acid sequence.
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Reaction Mechanism of Fluoroacetate Dehalogenase from Moraxella sp. B
Ji Quan Liu,Tatsuo Kurihara,Susumu Ichiyama,Masaru Miyagi,Susumu Tsunasawa,Haruhiko Kawasaki,Kenji Soda,Nobuyoshi Esaki +7 more
TL;DR: Results suggest that His-272 probably acts as a base catalyst for the hydrolysis of the covalent ester intermediate in the dehalogenation of fluoroacetate and other haloacetates.
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Purification and properties of two type-B α-l-arabinofuranosidases produced by Penicillium chrysogenum
TL;DR: Two distinct extracellular alpha-L-arabinofuranosidases (AFases; EC 3.55) were purified from the culture filtrate of Penicillium chrysogenum 31B and displayed broad substrate specificities; they released arabinose from branched arabinan, debranchedArabinan), arabinoxylan, arabinogalactan, and arabino-oligosaccharides.
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Purification and Properties of Haloacetate Halidohydrolase Specified by Plasmid from Moraxella sp. Strain B
TL;DR: Haloacetate halidohydrolase II specified by a plasmid pUO1 was purified from halo acetate-assimilating Moraxella sp.