The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca 2+ dependency and specific activity.

.alpha.-amylase mutants

The present invention concerns cellulase-containing granular detergent compositions which are in a "compact" form, i.e. they are of a relatively high density and contain a relatively low amount of inorganic filler salt compared to conventional detergent compositions. In the detergent compositions herein the cellulase is defined by the C14CMC method described herein and preferably comprises a specific single-component endoglucanase.

Compact detergent compositions with high activity cellulase

An α-amylase characterized by having a specific activity at least 25 % higher than the specific activity of Termamyl® at a temperature in the range of 25 °C to 55 °C and at a pH value in the range of pH 8 to pH 10.

Alkaline bacillus amylase

An enzyme composition with substantial cellulolytic, especially endoglucanase, activity at alkaline conditions which is derived from or producible by fungi from the genus Acremonium, provided that the enzyme composition is not immunologically cross-reactive with antibodies raised against endoglucanases (EC 3.2.1.4) from Humicola insolens, DSM 1800, Fusarium oxysporum, DSM 2672, Myceliophthora thermophila, CBS 117.65, or Cephalosporium sp., RYM-202. The invention further relates to a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity. The enzyme composition and the isolated or cloned and expressed cellulase of the invention may be useful in any industrial process requiring an alkaline cellulase, e.g. for providing localised variation in the colour density of dyed fabric such as stone-washing of denim, for improving the drainage of an aqueous suspension of paper pulp, for the de-inking of recycled paper, in detergent compositions and in fabric softeners.

An enzyme and enzyme preparation with endoglucanase activity

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

A cellulase preparation consisting essentially of a homogeneous endoglucanase component which is immunoreactive with an antibody raised against a highly purified ∩43kD endoglucanase derived from Humicola insolens, DSM 1800, or which is homogeneous to said ∩43kD endoglucanase, may be employed in the treatment cellulose-containing fabrics for harshness reduction or colour clarification or to provide a localized variation in the colour of such fabrics, or it may be employed in the treatment of paper pulp.

A cellulase preparation comprising an endoglucanase enzyme

A cellulose- or hemicellulose-degrading enzyme which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises amino acid sequence (α) or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.

An enzyme capable of degrading cellulose or hemicellulose.

A cellulose- or hemicellulose-degrading enzyme which is derivable from a fungus other than Trichoderma or Phanero-chaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises amino acid sequence (.alpha.) or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.

Enzyme capable of degrading cellulose or hemicellulose

PROBLEM TO BE SOLVED: To provide a new endoglucanase. SOLUTION: The cellulase preparation contains a homogeneous endoglucanase component having immunoreactivity with an antibody produced against a highly purified endoglucanase having a molecular weight of <=43 kD and derived from Humicola insolens DSM1800 or a derivative of the endoglucanase of <=43 kD molecular weight as an essential component.

Hastrup Sven

Papers

A cellulase preparation comprising an endoglucanase enzyme

An enzyme capable of degrading cellulose or hemicellulose.

Enzyme capable of degrading cellulose or hemicellulose

Cellulase preparation containing endoglucanase