H
Helen R. Wilson
Publications - 8
Citations - 640
Helen R. Wilson is an academic researcher. The author has contributed to research in topics: Transcription (biology) & Homologous recombination. The author has an hindex of 7, co-authored 7 publications receiving 592 citations.
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Journal ArticleDOI
Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Lynn C. Thomason,Donald L. Court,Mikail Bubunenko,Nina Costantino,Helen R. Wilson,Simanti Datta,Amos B. Oppenheim +6 more
TL;DR: Support protocols are presented that describe several two‐step selection/counter‐selection methods of making genetic alterations without leaving any unwanted changes in the targeted DNA, and a method for retrieving onto a plasmid a genetic marker from the Escherichia coli chromosome or a co‐electroporated DNA fragment.
Journal ArticleDOI
Mini-λ: a tractable system for chromosome and BAC engineering
Donald L. Court,Sundaram Swaminathan,Daiguan Yu,Helen R. Wilson,Teresa Baker,Mikail Bubunenko,James A. Sawitzke,Shyam K. Sharan +7 more
TL;DR: The ability to generate recombinants very efficiently demonstrates the usefulness of the mini-lambda as a very simple mobile system for in vivo genome engineering by homologous recombination, a process named recombineering.
Journal ArticleDOI
UNIT 1.16 Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination
Lynn C. Thomason,Donald L. Court,Mikail Bubunenko,Nina Costantino,Helen R. Wilson,Simanti Datta,Amos B. Oppenheim +6 more
TL;DR: In this paper, the authors describe a two-step method of making genetic alterations without leaving any unwanted changes, and a method for retrieving a genetic marker (cloning) from the E. coli chromosome or a coelectroporated DNA fragment and moving it onto a plasmid.
Journal ArticleDOI
The global regulator RNase III modulates translation repression by the transcription elongation factor N
TL;DR: It is shown that cleavage of the N leader by RNase III does not inhibit antitermination but prevents N‐mediated translation repression of N gene expression, which activates N gene translation at least 200‐fold.
Journal ArticleDOI
Translational repression by a transcriptional elongation factor.
TL;DR: This work demonstrates that N has an additional, hitherto unknown regulatory role, as a repressor of the translation of its own gene, and identifies one nut and several host mutations that eliminate antitermination and not translational repression, suggesting the independence of these two N-mediated mechanisms.