Helmut Merk

TL;DR: The synthesis of these two anti-lysozyme single-chain antibodies in high yields and with high biological activities in a cell-free E. coli expression system can provide significant amounts of various scFv fragments regardless of the order of variable regions, including those which are hardly expressed in vivo.

TL;DR: This work states that cell-free protein expression systems, in particular those of eukaryotic origin, have recently been developed as promising tools for the rapid and efficient production of a wide variety of membrane proteins, however, a large number of these proteins require posttranslational modifications for optimum function.

TL;DR: A eukaryotic cell-free system based on Spodoptera frugiperda cells was developed for the convenient synthesis of Fab antibody fragments and other disulfide bridge containing proteins, compatible with PCR-generated linear templates enabling automated generation of antibody fragments in a high-throughput manner.

TL;DR: The results indicate a stoichiometric incorporation of the azido amino acid at the desired position within the proteins, and the presented approach should be particularly useful for providing eukaryotic and membrane-associated proteins for investigation by fluorescence-based techniques.

TL;DR: Strong evidence is provided that membrane insertion of integral membrane proteins can represent a prime limiting factor for their preparative scale in vitro production of problematic membrane proteins in cell-free lysates in combination with supplied membranes.