Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infection, drug development, and clinical diagnosis. Gene detection has a wide range of applications, such as environmental, biomedical, pharmaceutical, agriculture and forensic medicine to name a few. Compared with Sanger sequencing, high-throughput sequencing technology has the advantages of larger output, high resolution, and low cost which greatly promotes the application of sequencing technology in life science research. Magnetic nanoparticles, as an important part of nanomaterials, have been widely used in various applications because of their good dispersion, high surface area, low cost, easy separation in buffer systems and signal detection. Based on the above, the application of magnetic nanoparticles in nucleic acid detection was reviewed.

/pdf/application-of-magnetic-nanoparticles-in-nucleic-acid-1k975eihep.pdf

Application of magnetic nanoparticles in nucleic acid detection

Chemical, physical, and biological coordination: An interplay between materials and enzymes as potential platforms for immobilization

Fundamental importance of ionic interactions in the liquid phase: A review of recent studies of ionic liquids in biomedical and pharmaceutical applications

Enzymes are natural catalysts highly specific to the substrate type and operate under mild conditions of temperature, pressure, and pH with high conversion rates, which makes them more efficient than conventional chemical catalysts. The enzymes can be obtained from various sources, animal, vegetable, and microbiological. Lipases are very versatile enzymes, and this has aroused the interest of the industries. However, the great problem of the use of soluble lipases is the high cost of acquisition, low operational stability, and difficulties of recovery, and reuse. Enzymatic immobilization has been suggested as an alternative to reduce the limitations of soluble enzymes, increasing their stability and facilitating recovery, and reuse, significantly reducing the cost of processes involving the use of enzymes. This review presents a discussion on the different immobilization methods for lipase, as well as the challenges of use lipases immobilized on the industrial scale.

Lipases: sources, immobilization methods, and industrial applications.

Biopolymer processing and handling is greatly facilitated by the use of ionic liquids, given the increased solubility, and in some cases, structural stability imparted to these molecules. Focussing on proteins, we highlight here not just the key drivers behind protein-ionic liquid interactions that facilitate these functionalities, but address relevant current and potential applications of protein-ionic liquid interactions, including areas of future interest.

/pdf/proteins-in-ionic-liquids-reactions-applications-and-futures-10diu6kya8.pdf

Proteins in Ionic Liquids: Reactions, Applications, and Futures

Ionic liquids-modified cellulose coated magnetic nanoparticles for enzyme immobilization: Improvement of catalytic performance.

Synthesis of functional ionic liquid modified magnetic chitosan nanoparticles for porcine pancreatic lipase immobilization

Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent.

Enhancement of catalytic performance of porcine pancreatic lipase immobilized on functional ionic liquid modified Fe3O4-Chitosan nanocomposites

In this study, a novel enzyme immobilization method was developed to enhance the catalytic stability of enzymes. In this strategy, ionic liquid (IL) modified magnetic chitosan (MCS) composites were used as supports for lipase adsorption and graphene oxide (GO) was employed as shell coating for the first time. The modifier used was imidazolium-based IL with a side alkyl chain which was composed of 8 −CH2 and a terminal hydroxyl group. The prepared supports IL-MCS, immobilized lipase PPL-IL-MCS, and GO/PPL-IL-MCS were well characterized. The GO shielding lipase GO/PPL-IL-MCS maintained high activity (2468 U/g), which was 6.72-fold of free lipase. In addition, the pH and temperature effect on lipase activity were investigated. The thermal stability, denaturants stability, storage stibility, and reusing stability were also studied. Compared to PPL-IL-MCS, the stabilities of GO/PPL-IL-MCS were all enhanced while keeping high activity. For example, after 10 cycles of reuse, the residual activity of GO/PPL-IL-MC...

Hongbo Suo

Papers

Ionic liquids-modified cellulose coated magnetic nanoparticles for enzyme immobilization: Improvement of catalytic performance.

Synthesis of functional ionic liquid modified magnetic chitosan nanoparticles for porcine pancreatic lipase immobilization

Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent.

Enhancement of catalytic performance of porcine pancreatic lipase immobilized on functional ionic liquid modified Fe3O4-Chitosan nanocomposites

Graphene Oxide Nanosheets Shielding of Lipase Immobilized on Magnetic Composites for the Improvement of Enzyme Stability