The influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition. 
The isotopic composition of the nitrogen in an animal reflects the nitrogen isotopic composition of its diet. The δ^(15)N values of the whole bodies of animals are usually more positive than those of their diets. Different individuals of a species raised on the same diet can have significantly different δ^(15)N values. The variability of the relationship between the δ^(15)N values of animals and their diets is greater for different species raised on the same diet than for the same species raised on different diets. Different tissues of mice are also enriched in ^(15)N relative to the diet, with the difference between the δ^(15)N values of a tissue and the diet depending on both the kind of tissue and the diet involved. The δ^(15)N values of collagen and chitin, biochemical components that are often preserved in fossil animal remains, are also related to the δ^(15)N value of the diet. 
The dependence of the δ^(15)N values of whole animals and their tissues and biochemical components on the δ^(15)N value of diet indicates that the isotopic composition of animal nitrogen can be used to obtain information about an animal's diet if its potential food sources had different δ^(15)N values. The nitrogen isotopic method of dietary analysis probably can be used to estimate the relative use of legumes vs non-legumes or of aquatic vs terrestrial organisms as food sources for extant and fossil animals. However, the method probably will not be applicable in those modern ecosystems in which the use of chemical fertilizers has influenced the distribution of nitrogen isotopes in food sources. 
The isotopic method of dietary analysis was used to reconstruct changes in the diet of the human population that occupied the Tehuacan Valley of Mexico over a 7000 yr span. Variations in the δ^(15)C and δ^(15)N values of bone collagen suggest that C_4 and/or CAM plants (presumably mostly corn) and legumes (presumably mostly beans) were introduced into the diet much earlier than suggested by conventional archaeological analysis.

Influence of Diet On the Distribtion of Nitrogen Isotopes in Animals

Rising atmospheric carbon dioxide (CO2), primarily from human fossil fuel combustion, reduces ocean pH and causes wholesale shifts in seawater carbonate chemistry. The process of ocean acidification is well documented in field data, and the rate will accelerate over this century unless future CO2 emissions are curbed dramatically. Acidification alters seawater chemical speciation and biogeochemical cycles of many elements and compounds. One well-known effect is the lowering of calcium carbonate saturation states, which impacts shell-forming marine organisms from plankton to benthic molluscs, echinoderms, and corals. Many calcifying species exhibit reduced calcification and growth rates in laboratory experiments under high-CO2 conditions. Ocean acidification also causes an increase in carbon fixation rates in some photosynthetic organisms (both calcifying and noncalcifying). The potential for marine organisms to adapt to increasing CO2 and broader implications for ocean ecosystems are not well known; both are high priorities for future research. Although ocean pH has varied in the geological past, paleo-events may be only imperfect analogs to current conditions.

/pdf/ocean-acidification-the-other-co-2-problem-4h18mw2cpj.pdf

Ocean Acidification: The Other CO 2 Problem

https://homepages.uc.edu/~algeot/Tribovillard-Algeo-etal-CG-2006.pdf

Trace metals as paleoredox and paleoproductivity proxies: An update

We used 5704 14C, 10Be, and 3He ages that span the interval from 10,000 to 50,000 years ago (10 to 50 ka) to constrain the timing of the Last Glacial Maximum (LGM) in terms of global ice-sheet and mountain-glacier extent. Growth of the ice sheets to their maximum positions occurred between 33.0 and 26.5 ka in response to climate forcing from decreases in northern summer insolation, tropical Pacific sea surface temperatures, and atmospheric CO2. Nearly all ice sheets were at their LGM positions from 26.5 ka to 19 to 20 ka, corresponding to minima in these forcings. The onset of Northern Hemisphere deglaciation 19 to 20 ka was induced by an increase in northern summer insolation, providing the source for an abrupt rise in sea level. The onset of deglaciation of the West Antarctic Ice Sheet occurred between 14 and 15 ka, consistent with evidence that this was the primary source for an abrupt rise in sea level ~14.5 ka.

https://science.sciencemag.org/content/sci/325/5941/710.full.pdf

The Last Glacial Maximum.

Changing the strength of connections between neurons is widely assumed to be the mechanism by which memory traces are encoded and stored in the central nervous system. In its most general form, the synaptic plasticity and memory hypothesis states that "activity-dependent synaptic plasticity is induced at appropriate synapses during memory formation and is both necessary and sufficient for the infor- mation storage underlying the type of memory mediated by the brain area in which that plasticity is observed." We outline a set of criteria by which this hypothesis can be judged and describe a range of experimental strategies used to investigate it. We review both classical and newly discovered properties of synaptic plasticity and stress the importance of the neural architecture and synaptic learning rules of the network in which it is embedded. The greater part of the article focuses on types of memory mediated by the hippocampus, amygdala, and cortex. We conclude that a wealth of data supports the notion that synaptic plasticity is necessary for learning and memory, but that little data currently supports the notion of sufficiency.

Synaptic plasticity and memory: an evaluation of the hypothesis

Magnesium/calcium data from planktonic foraminifera in equatorial Pacific sediment cores demonstrate that tropical Pacific sea surface temperatures (SSTs) were 2.8° ± 0.7°C colder than the present at the last glacial maximum. Glacial-interglacial temperature differences as great as 5°C are observed over the last 450 thousand years. Changes in SST coincide with changes in Antarctic air temperature and precede changes in continental ice volume by about 3 thousand years, suggesting that tropical cooling played a major role in driving ice-age climate. Comparison of SST estimates from eastern and western sites indicates that the equatorial Pacific zonal SST gradient was similar or somewhat larger during glacial episodes. Extraction of a salinity proxy from the magnesium/calcium and oxygen isotope data indicates that transport of water vapor into the western Pacific was enhanced during glacial episodes.

https://science.sciencemag.org/content/sci/289/5485/1719.full.pdf

Climate Impact of Late Quaternary Equatorial Pacific Sea Surface Temperature Variations

Cultured planktonic foraminifera, Orbulina universa (symbiotic) and Globigerina bulloides (nonsymbiotic), are used to reexamine temperature:δ18O relationships at 15°–25°C. Relationships for both species can be described by linear equations. Equations for O. universa grown under low light (LL) and high light (HL) share a slope of −4.80 (0.21‰ °C−1) with a HL-LL offset of −0.33‰ due to symbiont photosynthetic activity. The effect of [CO32−] on O. universa is −0.002‰ µmol−1 kg−1 and is insensitive to temperature. For G. bulloides, ontogenetic effects produce size-related trends in temperature:δ18O, whereby larger shells are enriched in 18O relative to smaller specimens. The O. universa temperature:δ18O relationships are more accurate than previously published equations for describing plankton tow data. Our equations do not explain planktonic core top data with the same precision but provide a good fit to benthic Cibicidoides data below 10°C. Temperature:δ18O relationships for G. bulloides provide good agreement with field data for this species from the northeast Pacific.

https://agupubs.onlinelibrary.wiley.com/doi/pdf/10.1029/98PA00070

Reevaluation of the oxygen isotopic composition of planktonic foraminifera: Experimental results and revised paleotemperature equations

Stable oxygen and carbon isotope measurements on biogenic calcite and aragonite have become standard tools for reconstructing past oceanographic and climatic change. In aquatic organisms, 18O/16O ratios in the shell carbonate are a function of the ratio in the sea water and the calcification temperature1. In contrast, 13C/12C ratios are controlled by the ratio of dissolved inorganic carbon in sea water and physiological processes such as respiration and symbiont photosynthesis2. These geochemical proxies have been used with analyses of foraminifera shells to reconstruct global ice volumes3, surface and deep ocean temperatures4,5, ocean circulation changes6 and glacial–interglacial exchange between the terrestrial and oceanic carbon pools7. Here, we report experimental measurements on living symbiotic and non-symbiotic plankton foraminifera (Orbulina universa and Globigerina bulloides respectively) showing that the 13C/12C and 18O/16O ratios of the calcite shells decrease with increasing seawater [CO32−]. Because glacial-period oceans had higher pH and [CO32−] than today8, these new relationships confound the standard interpretation of glacial foraminiferal stable-isotope data. In particular, the hypothesis that the glacial–interglacial shift in the 13C/12C ratio was due to a transfer of terrestrial carbon into the ocean7 can be explained alternatively by an increase in ocean alkalinity25. A carbonate-concentration effect could also help explain some of the extreme stable-isotope variations during the Proterozoic and Phanerozoic aeons9.

/pdf/effect-of-seawater-carbonate-concentration-on-foraminiferal-48xq21tel2.pdf

Effect of seawater carbonate concentration on foraminiferal carbon and oxygen isotopes

Stable oxygen and carbon isotope measurements on biogenic calcite and aragonite have become standard tools for reconstructing past oceanographic and climatic change. In aquatic organisms, 18O/16O ratios in the shell carbonate are a function of the ratio in the sea water and the calcification temperature. In contrast, 13C/12C ratios are controlled by the ratio of dissolved inorganic carbon in sea water and physiological processes such as respiration and symbiont photosynthesis. These geochemical proxies have been used with analyses of foraminifera shells to reconstruct global ice volumes, surface and deep ocean temperatures,, ocean circulation changes and glacial–interglacial exchange between the terrestrial and oceanic carbon pools. Here, we report experimental measurements on living symbiotic and non-symbiotic plankton foraminifera (Orbulina universa and Globigerina bulloides respectively) showing that the 13C/12C and 18O/16O ratios of the calcite shells decrease with increasing seawater [CO32−]. Because glacial-period oceans had higher pH and [CO32−] than today, these new relationships confound the standard interpretation of glacial foraminiferal stable-isotope data. In particular, the hypothesis that the glacial–interglacial shift in the 13C/12C ratio was due to a transfer of terrestrial carbon into the ocean can be explained alternatively by an increase in ocean alkalinity. A carbonate-concentration effect could also help explain some of the extreme stable-isotope variations during the Proterozoic and Phanerozoic aeons.

Howard J. Spero

Papers

Climate Impact of Late Quaternary Equatorial Pacific Sea Surface Temperature Variations

Reevaluation of the oxygen isotopic composition of planktonic foraminifera: Experimental results and revised paleotemperature equations

Effect of seawater carbonate concentration on foraminiferal carbon and oxygen isotopes

Effect of seawater carbonate concentration on foraminiferal carbon and oxygen isotopes

Controls on magnesium and strontium uptake in planktonic foraminifera determined by live culturing