The invention discloses a flos chrysanthemi indici effective ingredient, which is extracted by a method comprising the following steps of: 1) taking flos chrysanthemi indici, adding 60-90% ethanol which is 5-10 times of the weight of the flos chrysanthemi indici to extract, filtering the extracting solution, and concentrating the filtrate into extract; and 2) adding water into the extract to prepare suspension, adding extract liquor to extract, and recovering the extract liquor to obtain the flos chrysanthemi indici effective ingredient. Experiments show that the flos chrysanthemi indici effective ingredient can more obviously inhibit hepatitis B virus, can also inhibit the glutamic-oxalacetic transaminease (AST) and glutamic-pyruvic transaminease (ALT) indexes of liver serum of the damaged liver, can reduce the tumor necrosis factor (TNF) content, liver indexes and malondialdehyde content of the liver serum of the damaged liver, and has good liver protection function. The flos chrysanthemi indici effective ingredient can be used as the active ingredient, and can be prepared into anti-hepatitis B virus medicaments or compositions with the liver protection function, such as food, drinks, health care products, drugs and the like, and the medicaments or compositions can be prepared into tablet, capsule, powder, injections and any other medically acceptable formulations.

Flos chrysanthemi indici effective ingredient and application thereof

The invention discloses a poria cocos extract, which contains 40 to 90 percent of poria cocos triterpene. The poria cocos extract is prepared by a method comprising the following steps of crushing poria cocos; adding a proper amount of ethanol solution; performing extraction under the heating conditions; performing suction filtration to obtain filter liquid; merging filter liquid; performing concentration; adding a small amount of water; performing extraction by ethyl acetate for many times; performing reduced pressure drying; performing dissolution by methanol; performing filtering; performing reduced pressure concentration on filter liquid; performing freeze drying; obtaining the solid state poria cocos extract. The poria cocos extract prepared by the method is from traditional Chinese medicine; results of in vitro experiments show that the extract has anti-tumor activity and can reverse the multi-drug drug resistance of MCF/ADR.

Preparation of poria cocos extract capable of reversing tumor multi-drug drug resistance

The invention discloses a hydroxyethylation method capable of improving polysaccharide immunity activity of hericium erinaceus. The hydroxyethylation method capable of improving polysaccharide immunity activity of hericium erinaceus comprises the following steps: dissolving hericium erinaceus polysaccharide in a mixed solution of sodium hydroxide and sodium chloride, and stirring and dissolving; cooling the obtained solution to be at the temperature of 0 DEG C, feeding nitrogen and discharging air, then dropwise adding ethylene oxide and stirring and reacting under the condition of ice bath; after reaction is finished, heating the obtained solution to the room temperature, and stirring overnight; neutralizing the solution until the solution is neutral in the next day, adding ethanol and acetone, carrying out centrifugal separation after white precipitate appears, dissolving the precipitate with water, and then carrying out dialysis and freeze drying to obtain hydroxyethylated hericiumerinaceus polysaccharide. The hericium erinaceus polysaccharide is subjected to hydroxyethylation modification, thus, the immunocompetence of the hericium erinaceus polysaccharide is improved, by theprepared hericium erinaceus polysaccharide, the antigen presentation capability of DCs can be improved remarkably, and DCs cell secretion IL-12, IL-18 and IL-15 is promoted.

Hydroxyethylation method capable of improving polysaccharide immunity activity of hericium erinaceus

The invention discloses application of tuckahoe triterpenes to preparation of a liver-protecting medicine or health care food. The total tuckahoe triterpenes are prepared by ethanol soaking and ethyl acetate extraction. A carbon tetrachloride-induced mouse liver injury model is selected to preliminarily study the liver-protecting effect of the total tuckahoe triterpenes, and a serum index detection result proves that the tuckahoe triterpenes have the effect of protecting the damaged liver; in addition, the liver fibrosis lesion of a treatment group is improved and dose-related according to a liver tissue pathological section. Tuckahoe serving as the health care food with the liver-protecting effect has wide application and development prospects.

Application of tuckahoe triterpenes to preparation of liver-protecting medicine

The invention discloses a preparation method of hydroxyethyl poria cocos polysaccharide and an application of the hydroxyethyl poria cocos polysaccharide as a framework material of a sustained release tablet. According to the preparation method disclosed by the invention, dialysate after a hydroxyethyl poria cocos polysaccharide reaction is concentrated through an ultra filtration membrane, or spray drying is directly performed on a concentrated solution of the hydroxyethyl poria cocos polysaccharide, so that hydroxyethyl poria cocos polysaccharide powder is obtained. The hydroxyethyl poria cocos polysaccharide powder has favorable fluidity, compressibility and adhesiveness, and not only is suitable for direct tabletting of powder but also is suitable for tabletting through wet granulation. Medicine tablets prepared by the hydroxyethyl poria cocos polysaccharide can swell to form gel in artificial gastrointestinal fluid, little medicine release is released in artificial gastric fluid, but in artificial intestinal fluid, medicines have notable slow release effects, diffusion is used as a main medicine release manner, and besides, the erosion of a gel layer is accompanied, and the hydroxyethyl poria cocos polysaccharide is suitable for being used as the framework material of the sustained-release tablet of various medicines particularly polysaccharide protein type medicines and other water soluble medicines. The medicine release speed can be adjusted by changing the substitution degree and the molecular weight of the hydroxyethyl poria cocos polysaccharide, or by adding other auxiliary materials.

Preparation of hydroxyethyl poria cocos polysaccharide and application of hydroxyethyl poria cocos polysaccharide as framework material of sustained release tablet

Hu Xianming

Papers

Application of tuckahoe triterpenes to preparation of liver-protecting medicine

Preparation of hydroxyethyl poria cocos polysaccharide and application of hydroxyethyl poria cocos polysaccharide as framework material of sustained release tablet

2, 7-bi-substituted fluorenone derivative and preparation method and application thereof

7,4'-disubstituted isoflavone derivative and preparation method and application thereof