H
Hubertus Hohenblum
Researcher at University of Natural Resources and Life Sciences, Vienna
Publications - 6
Citations - 674
Hubertus Hohenblum is an academic researcher from University of Natural Resources and Life Sciences, Vienna. The author has contributed to research in topics: Pichia pastoris & Unfolded protein response. The author has an hindex of 5, co-authored 6 publications receiving 640 citations.
Papers
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Journal ArticleDOI
Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris.
TL;DR: An increase of BiP was observed upon induction of expression, indicating that the intracellular product fraction exerts an unfolded protein response in the host cells.
Journal ArticleDOI
Stress in recombinant protein producing yeasts.
TL;DR: Apart from discussing well-documented stress reactions of yeasts in the context of heterologous protein production, some more speculative topics like quorum sensing and apoptosis are addressed.
Journal ArticleDOI
Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry.
TL;DR: Data demonstrate that flow cytometry is a powerful tool for the analysis and optimization of recombinant protein production processes, and they indicate the need to further improve a widely used fermentation protocol for P. pastoris.
Journal ArticleDOI
Bacterial expression and refolding of human trypsinogen
TL;DR: This work describes the high level expression of human trypsinogen 1 in E. coli using the T7 expression system and the utility for the detachment of mammalian cells in culture was proven.
Patent
Method for the manufacture of recombinant trypsin
TL;DR: In this article, a method for the manufacture and purification of recombinant trypsinogen and triedpsin in E. coli and yeast, using high yield expression vectors with and without secretion leader sequences, was described.