Showing papers in "Journal of Biotechnology in 2003"

TL;DR: These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil.

TL;DR: Six bacterial strains with the capability of degrading textile dyes were isolated from sludge samples and mud lakes and Aeromonas hydrophila was selected and identified because it exhibited the greatest color removal from various dyes.

TL;DR: The growing market for amino acids produced with Corynebacteria led to significant improvements in bioprocess and downstream technology as well as in molecular biology, and one focus of this review is on recent developments in downstream technology.

TL;DR: The toxicity of C.I. Reactive Black 5 and three Procion dyes, as found in textile effluents, was determined using the bioluminescent bacterium Vibrio fischeri using a baffled bioreactor and no genotoxicity was detected after the decolourisation of either the parent or the hydrolysed reactive dyes.

TL;DR: A simple PCR method utilizing a water-in-oil (W/O) emulsion that included numerous droplets of reaction mixture in bulk oil phase that allows the effective concentration of template DNA to be increased, even for low concentrations of templateDNA.

TL;DR: The objectives of this study are to summarize the published literature on tools for genetic engineering in C. glutamicum and to focus on new sophisticated and highly efficient methods in the fields of DNA transfer techniques, cloning vectors, integrative genetic tools, and antibiotic-free self-cloning.

TL;DR: Recent and future biotechnological research on certain key myxobacteria and on their ability to produce natural products is reviewed with the focus on the production of myxovirescin, soraphen and epothilone.

TL;DR: This work examined the anaerobic digestion of wastewater sludge using a clostridium strain isolated from the sludge as inoculum and obtained a much higher hydrogen yield than presented in the literature.

TL;DR: Examination of the mechanism of the decolorization process by cell beads indicated that it proceeded primarily by biologicalDecolorization associated with partial adsorption of the dye onto the entrapped cells and gel matrix.

TL;DR: Sericin was completely removed from the warp yarns of the crêpe fabric, while the highly twisted weft yarns still exhibited the presence of sericin deposits within the most internal parts of the close fibre texture.

TL;DR: Ethanol was produced by simultaneous saccharification and fermentation (SSF) from beech wood chips after bioorganosolve pretreatments by ethanolysis and white rot fungi, Ceriporiopsis subvermispora, which gave the highest yield on SSF.

TL;DR: The phenotype of defined mutants revealed that the bc(1)-aa(3) branch, but not the bd branch, is of major importance for aerobic growth in minimal medium and the system of oxidative phosphorylation represents an attractive target for improving amino acid productivity of C. glutamicum by metabolic engineering.

TL;DR: This review summarizes the present knowledge on the different steps of the fundamental pathways of acetate utilization in C. glutamicum, namely, on acetate transport, acetate activation, tricarboxylic acid (TCA) cycle, glyoxylate cycle and gluconeogenesis.

TL;DR: This review summarizes the current knowledge on the diazotrophic endophyte Azoarcus sp.

TL;DR: The results show that the removal of carbon and nitrogen compounds was accomplished efficiently by using various kinds of bacteria distributed vertically and horizontally in a single reactor.

TL;DR: Different mechanisms of nitrogen-control of the synthesis of exocellular polysaccharide are reported for each strain, which results in the requirement of a species-specific optimisation of the cultivation conditions for the development of an efficient technology for the production of cyanobacterial exopolysaccharides.

TL;DR: The results suggest a possible use of the transglutaminase polymerized pectin-soy protein films as edible food or drug coatings.

TL;DR: The proposed model provides good insights into the thermodynamic mechanisms of biosorption of heavy metals and may imply that aerobic granules are effective biosorbent for the removal of Cd(2+), Cu(2+) and Zn( 2+) from industrial wastewater.

TL;DR: Observations suggest that C. glutamicum promoters functionally conform with the common bacterial promoter scheme, although they differ in some sequence structures.

TL;DR: It was possible to determine the degree of direct incorporation of the lipophilic substrates into the sophorolipids for a homologous series of alkanes, and the amounts of these crystals recovered were significantly higher than those observed for any of the oily products.

TL;DR: The results indicate that subtractive SELEX is a useful tool in finding ligands to specific biological markers that distinguish a subtype of cells from cells of homologous origin, such as carcinoma cells among normal epithelial tissues.

TL;DR: The GC-MS protocol as well as the new algorithm demonstrated here proved to be a powerful tool for characterizing metabolic regulation and can be utilized for strain improvement and bioprocess optimization.

TL;DR: It was demonstrated that complete organics and nitrogen removal can be achieved in single granule-based SBR with high efficiency and stable performance and is the first study to show the capability of microbial granules in simultaneous removal of organic carbon and nitrogen from wastewater.

TL;DR: Immunostimulatory effects of isolates were tested on induction of cytokine (tumour necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) synthesis in primary cultures of human peripheral blood mononuclear cells (PBMC) isolated from a buffy coat.

TL;DR: In this paper, the possible use of olive-mill wastewater (OMW) as a growth medium for the production of extracellular laccase and manganese peroxidase (MnP) from the white-rot fungus Panus tigrinus was studied using a properly formulated OMW-based medium (2-fold diluted OMW supplemented with 0.5% sucrose and 0.1% yeast extract).

TL;DR: An overview of the strategy for the identification and characterization of numerous biosynthesis gene clusters for polyketides displaying interesting functions and particular structural features is given.

TL;DR: A fusion protein composed of a cellulose-binding module (CBM) from Neocallimastix patriciarum cellulase 6A and lipase B from Candida antarctica (CALB) was produced by Pichia pastoris Mut(+) in high-cell density bioreactor cultures and obtained full-length protein with a considerably higher purity in the culture supernatant compared with the original cultivation.

TL;DR: C-phycocyanin was purified from Spirulina (Arthrospira) fusiformis by a multi-step treatment of the crude extract with rivanol in a ratio 10:1 (v/v), followed by 40% saturation with ammonium sulfate, and had an emission and absorption maxima at 620 and 650 nm, respectively and absorbance ratio A(620)/A(280).

TL;DR: A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions to carry out magnetic separation of DNA.

TL;DR: The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no disease symptoms develop indicating that all genes required for successful infection, establishment and growth in the plant reside on the chromosome.