H
Huoqing Huang
Researcher at Civil Aviation Authority of Singapore
Publications - 173
Citations - 4216
Huoqing Huang is an academic researcher from Civil Aviation Authority of Singapore. The author has contributed to research in topics: Pichia pastoris & Xylanase. The author has an hindex of 35, co-authored 143 publications receiving 3506 citations.
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Journal ArticleDOI
Improved thermal performance of Thermomyces lanuginosus GH11 xylanase by engineering of an N-terminal disulfide bridge.
TL;DR: It is shown that N-terminal disulfide bridges can stabilize even thermostable family GH11 xylanases, in contrast to the situation in acidic-neutral pH, which decreased the thermostability of xylanase in alkaline pH.
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A novel highly acidic β-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris
Huiying Luo,Yaru Wang,Hui Wang,Jun Yang,Yuhui Yang,Huoqing Huang,Peilong Yang,Yingguo Bai,Pengjun Shi,Yunliu Fan,Bin Yao +10 more
TL;DR: The cloned gene man5A had good pH adaptability, excellent thermal and pH stability, and high resistance to both pepsin and trypsin, and is a potential candidate for use in various industrial applications.
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Diversity of Beta-Propeller Phytase Genes in the Intestinal Contents of Grass Carp Provides Insight into the Release of Major Phosphorus from Phytate in Nature
TL;DR: Beta-propeller phytase (BPP) gene fragments were readily amplified from the intestinal contents of grass carp directly or from phytate-degrading isolates from the same source, confirming the widespread occurrence of BPP in aquatic communities and the first study to experimentally estimate BPP diversity in situ.
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Two xylose-tolerant GH43 bifunctional β-xylosidase/α-arabinosidases and one GH11 xylanase from Humicola insolens and their synergy in the degradation of xylan.
TL;DR: This study provides several enzymes for synergistic degradation of xylan and contributes to the formulation of optimised enzyme mixtures for the efficient hydrolysis of plant biomass.
Journal ArticleDOI
Thermostability Improvement of a Streptomyces Xylanase by Introducing Proline and Glutamic Acid Residues
Kun Wang,Huiying Luo,Jian Tian,Ossi Turunen,Huoqing Huang,Pengjun Shi,Huifang Hua,Caihong Wang,Shuanghe Wang,Bin Yao +9 more
TL;DR: Four residues expected to affect the thermostability of Streptomyces sp.