https://www.cell.com/trends/biotechnology/pdf/S0167-7799(10)00031-4.pdf

Recent Advances in Large-Scale Production of Monoclonal Antibodies and Related Proteins

Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia Although, the partition behavior involved in the method is complex and difficult to predict Current research shows that it has also been successfully used in the detection of veterinary drug residues in food, separation of precious metals, sewage treatment and a variety of other purposes The ATPS is able to give high recovery yield and is easily to scale up It is also very economic and environment friendly method The aim of this review is to overview the basics of ATPS, optimization and its applications

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Aqueous two-phase system (ATPS): an overview and advances in its applications.

Technology trends in antibody purification.

A steady increase of product titers and the corresponding change in impurity composition represent a challenge for development and optimization of antibody production processes. Additionally, increasing demands on product quality result in higher complexity of processes and analytics, thereby increasing the costs for product work-up. Concentration and composition of impurities are critical for efficient process development. These impurities can show significant variations, which primarily depend on culture conditions. They have a major impact on the work-up strategy and costs. The resulting "bottleneck" in downstream processing requires new optimization, technology and development approaches. These include the optimization and adaptation of existing unit operations respective to the new separation task, the assessment of alternative separation technologies and the search for new methods in process development. This review presents an overview of existing methods for process optimization and integration and indicates new approaches for future developments.

/pdf/trends-in-upstream-and-downstream-process-development-for-1oxg0xux6d.pdf

Trends in Upstream and Downstream Process Development for Antibody Manufacturing

General Principles and Strategies for Salting-Out Informed by the Hofmeister Series

Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals.

Affinity partitioning of human antibodies in aqueous two-phase systems

Affinity-enhanced purification of human antibodies by aqueous two-phase extraction

Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography.

I.F. Ferreira

Papers

Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals.

Affinity partitioning of human antibodies in aqueous two-phase systems

Affinity-enhanced purification of human antibodies by aqueous two-phase extraction

Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography.

Downstream processing of antibodies: single-stage versus multi-stage aqueous two-phase extraction.